- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M IHC, WB, ICC Rb Purified Polyclonal Antibody 描述 产品目录编号 AB9876 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Stargazin Antibody Alternate Names - Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)
产品信息 格式 Purified 演示 Liquid in 130 mM Tris-Cl, 87 mM Glycine, pH 7.4. 应用 应用 This Anti-Stargazin Antibody is validated for use in IP, WB, IC, IH for the detection of Stargazin. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Western blot: 0.1 μg/mL on Triton soluble fraction of rat brain. Suggested dilution buffer is TBS-T and blocking buffer is 3% skim milk. Suggested incubation time is 3 hours to overnight at room temperature.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 GST fusion protein from rat stargazin, cytoplasmic domain. 宿主 Rabbit 特异性 Stargazin [Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)]. By Western blot the antibody recognizes bands at ~35 kDa and ~55 kDa on Triton soluble fraction of rat brain. The antibody reacts dominantly with TARP gamma-8 and weakly with gamma-3 and gamma-4. Stargazin is expressed strongly in the hippocampus and cerebellum. 品种反应性 RatMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006078.2
Entrez基因汇总 L-type calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of several gamma subunit proteins. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This protein is similar to the mouse stargazin protein, mutations in which have been associated with absence seizures, also known as petit-mal or spike-wave seizures. This gene is a member of the neuronal calcium channel gamma subunit gene subfamily of the PMP-22/EMP/MP20 family. 基因符号 - MGC138502
- CACNG2
- stargazin
- MGC138504
纯化方法 Immunoaffinity purified UniProt编号 - Q9Y698
UniProt汇总 FUNCTION: SwissProt: Q9Y698 # Thought to stabilize the calcium channel in an inactivated (closed) state.
SIZE: 323 amino acids; 35966 Da
SUBUNIT: The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Interacts with the PDZ domains of DLG4/PSD-95 and DLG1/SAP97. May interact with GOPC (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain.
PTM: Phosphorylation of Thr-321 impairs interaction with DLG1 and DLG4.
SIMILARITY: SwissProt: Q9Y698 ## Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze thaw cycles. The antibody may be kept at 2-8°C for 1-3 weeks after thawing. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,ST1673,Anti-AFP Mouse mAb (1G7)
下一件merck millipore产品：merck millipore,默克密理博,AB15410,Anti-APG12 Antibody
相关产品
merck millipore,默克密理博,AB9487,Anti-Somatostatin Receptor Type 4 Antibody
merck millipore,默克密理博,AB5573-200UL,Anti-Urotensin II Receptor Antibody
merck millipore,默克密理博,70980,Trail Mix™ Protein Markers
merck millipore,默克密理博,F84-GL3,Manifold for CellASIC ONIX, for use with F84 plates
merck millipore,默克密理博,ST1673,Anti-AFP Mouse mAb (1G7)
merck millipore,默克密理博,AB15410,Anti-APG12 Antibody
merck millipore,默克密理博,07-648,Anti-Aurora A Antibody
merck millipore,默克密理博,AB939,Anti-Pancreatic Polypeptide Antibody
merck millipore,默克密理博,SF-818,miR-149-5p; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,0500-2270,Guava Flow Cell (easyCyte Systems)