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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9074 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-IP3 Receptor 2 Antibody Alternate Names - Inositol 1,4,5-Trisphosphate Receptor 2
产品信息 格式 Serum 控制 - Western blot = adult mouse liver.
IHC = adult mouse hepatocytes (adult mouse cerebellum Purkinje cells will be negative).
演示 Rabbit serum. Liquid. 应用 应用 Detect IP3 Receptor 2 using this Anti-IP3 Receptor 2 Antibody validated for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 2 protein in microsomal preparations. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel or 5% PAGE. For microsomal methods and blotting procedure similar antibodies can be found in: Perez, et al. J Biol. Chem. 272:23961
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from the C terminus of human IP3 Receptor 2. 宿主 Rabbit 特异性 IP3 Receptor 2. 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002223.2
基因符号 - IP3R2
- ITPR2
- InsP3R2
UniProt编号 - Q14571
UniProt汇总 FUNCTION: SwissProt: Q14571 # Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.
SIZE: 2701 amino acids; 308078 Da
SUBUNIT: Homotetramer (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: The short isoform is found in skeletal muscle and heart.
DOMAIN: SwissProt: Q14571 The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand- binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
PTM: Phosphorylated on tyrosine residues (By similarity).
SIMILARITY: Belongs to the InsP3 receptor family. & Contains 5 MIR domains.
MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium- binding protein which then inhibits the receptor.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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