- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5326 Replaces AB5922 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Nestin Antibody, clone 10C2 背景信息 Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma. 产品信息 格式 Purified 控制 - Positive Control: WI-38 (ATCC# CCL75) or U251 cell lines
演示 Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. 应用 应用 This Anti-Nestin Antibody, clone 10C2 is validated for use in IC, IH, IH(P), WB for the detection of Nestin. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunoblotting: 1:1,000-1:2,000 on denatured protein. Western blot: 1:1000-1:5000. Recognizes a 220-240kDA band on denaturing SDS gels. There is also a 60-64kDa band present in most lysates, the identity of this smaller fragment is unknown but it is not seen in lysates not containing human nestin protein.
WI-38 (ATCC# CCL75) can be used as a positive control. U251 cell line is also positive.
Immunocytochemistry: 1:200 on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton
Immunohistochemistry: paraformaldehyde is the preferred fixative: 1:200 on formalin fixed, paraffin embedded tissue sections. Use triton 0.02% in block only; dilution buffer 0.1% BSA; incubations 1-2 hours RT. Human fetal brain is suggested as a positive control. (CA Messam et al, Develop. Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002). Filamentous staining in the cytoplasm of cells
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Fusion protein. 表位 human 克隆 10C2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_006617.1
Entrez基因汇总 Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM] 基因符号 - Nbla00170
- NES
- ORFNames=Nbla00170
- FLJ21841
- Nestin.
非反应性品种 - Rat
- Mouse
纯化方法 Protein A purfied UniProt编号 - P48681
UniProt汇总 FUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).
SIZE: 1621 amino acids; 177439 Da
SUBUNIT: Interacts with FHOD3 (By similarity).
TISSUE SPECIFICITY: CNS stem cells.
DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Antibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,ABC106,Anti-RRM2 Antibody
下一件merck millipore产品：merck millipore,默克密理博,ABN485,Anti-SIPA1L1 Antibody
相关产品
merck millipore,默克密理博,MAB8053F-K,Anti-Adenovirus, FITC conjugated
merck millipore,默克密理博,219503,Cdk4 Inhibitor V - CAS 943746-57-4 - Calbiochem
merck millipore,默克密理博,AB10145,Anti-GFP Antibody
merck millipore,默克密理博,CMTI-1,EmbryoMax Embryonic Stem Cell Line - Strain 129/SVEV, passage 11
merck millipore,默克密理博,ABC106,Anti-RRM2 Antibody
merck millipore,默克密理博,ABN485,Anti-SIPA1L1 Antibody
merck millipore,默克密理博,LSKMAGG10,PureProteome™ Protein G Magnetic Bead System
merck millipore,默克密理博,AB9074,Anti-IP3 Receptor 2 Antibody
merck millipore,默克密理博,567630,Sodium Salicylate - CAS 54-21-7 - Calbiochem