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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 AB747 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Collagen Type III Antibody 产品信息 格式 Purified 演示 500ul containing of 0.15M NaCl, 10 mM sodium phosphate pH 7.5, with 0.1% mannitol and o.1% dextran as stabilizers contains no preservatives.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.应用 应用 This Anti-Collagen Type III Antibody is validated for use in ELISA, IF for the detection of Collagen Type III. 主要应用 - ELISA
不建议的应用 - Western Blotting
应用说明 ELISA 1:3,000
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Human placental collagen type III 宿主 Rabbit 特异性 The antibody reacts with native and heat denatured (non-reduced) human collagen type III. Cross reactions with other types of collagen under native conditions do occur. There is 10% cross reactivity with human collagen type I, 2% cross reactivity with human collagen type II, and 4% cross reactivity with human collagen type IV and V. There is no cross reactivity with other human plasma proteins under native ELISA conditions. Reactivity under denatured conditions has not been examined.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.品种反应性 Human 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000090.3
Entrez基因汇总 This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish] 基因符号 - FLJ34534
- COL3A1
- EDS4A
UniProt编号 - P02461
UniProt汇总 FUNCTION: SwissProt: P02461 # Collagen type III occurs in most soft connective tissues along with type I collagen.
SIZE: 1466 amino acids; 138564 Da
SUBUNIT: Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
DISEASE: SwissProt: P02461 # Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type III (EDS-III) [MIM:130020]; also known as benign hypermobility syndrome. Inheritance is autosomal dominant. EDS is characterized by joint laxity and hyperextensible skin. It is divided into nine different subtypes based on biochemical and clinical variations. & Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type IV (EDS-IV) [MIM:130050]. EDS-IV is the most severe form of the disease, in that it often produces life-threatening consequences, such as rupture of the arteries, bowel, or uterus. A variant form of EDS-IV is Gottron type acrogeria [MIM:201200]. The main characteristics are atrophy and mottled-type hyperpigmentation of the acral skin, resulting in an aged appearance. & Defects in COL3A1 may be a cause of aortic aneurysm [MIM:100070]. Aortic aneurysm consists of a dangerous ballooning of the aorta which is caused by a defect in the artery's wall.
SIMILARITY: SwissProt: P02461 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Antibody is stable for 12 months from date of receipt when stored at 2-8ºC. Remove any nondispersed aggregates by microcentrifugation prior to use. 包装信息 数量 500 µL
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