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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Chp, H IHC, WB, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB6005 Replaces AB8102 描述 Anti-MT1-MMP Antibody, catalytic domain Alternate Names - MMP14
- Membrane-type-1 matrix metalloproteinase
- Membrane-type matrix metalloproteinase 1
- matrix metalloproteinase 14 (membrane-inserted)
- matrix metallopeptidase 14 (membrane-inserted)
- MT1MMP
- MMP-14
背景信息 Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes degrade components of extracellular matrix (ECM), including collagens, fibronectin, laminin and other glycoproteins. MMPs play an important role in wound healing, apoptosis, development, angiogenesis, cancer metastases, and tissue remodeling. Most MMPs are secreted as inactive proproteins which are cleaved and activated; however, MT1-MMP (MMP-14) is a member of the membrane-type subfamily. Each member of this subfamily has a transmembrane domain suggesting these proteins are expressed at the cell’s surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of ECM components and thought to be an important tool for cellular remodeling of the ECM. It is also involved in endothelial cell migration, and although not fully characterized, MT1-MMP activity appears to modulate endothelial invasion, and formation of capillary tubes during angiogenesis (Galvez, 2001). It also appears to be a factor in monocyte recruitment during inflammation (Matias-Roman, 2005). MT1-MMP also activates MMP-2 protein, an activity involved in tumor invasion. 产品信息 格式 Affinity Purified 控制 - HT-1080 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-MT1-MMP Antibody, catalytic domain is an antibody against MT1-MMP for use in WB, IC, IH & IP. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Western Blot Analysis: 0.5 µg/mL from a previous lot detected MT1-MMP in 10 µg of human normal uterus tissue lysate.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected MT1-MMP in prostate carcinoma tissue.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated MT1-MMP from 500 µg of human normal uterus RIPA lysate.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the catalytic domain of human MT1-MMP. 表位 Catalytic domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes MT1-MMP (MMP-14). 品种反应性 ChimpanzeeHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with chimpanzee based on 100% sequence homology.
Equine, sheep, porcine, canine, and bovine (99% sequence homology). Mouse and rat (97% sequence homology). Orangutan (92% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004986.1
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq]. 基因符号 - MMP-X1
- MMP14
- MTMMP1
- MMP-14
- MT1MMP
- MT1-MMP
纯化方法 Affinity Purfied UniProt编号 - P50281
UniProt汇总 FUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface.
COFACTOR: Binds 1 zinc ion per subunit (by similarity). & Calcium (by similarity).
SIZE: 582 amino acids; 65884 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: In stromal cells of colon, breast, and head and neck.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 质量保证 Evaluated by Western Blot in HT-1080 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MT1-MMP in 10 µg of HT-1080 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,CC1057,MMP-7, human, recombinant
下一件merck millipore产品：merck millipore,默克密理博,UFC501024,Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane
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