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品种反应性 主要应用 宿主 格式 抗体类型 Po, H, R, Mk WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB6003 Replaces 04-1048 描述 Anti-MMP-2 Antibody, hinge region Alternate Names - neutrophil gelatinase
- Gelatinase A
- Matrix metalloproteinase-2
- collagenase type IV-A
- matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase,<br />72kDa type IV collagenase)
- matrix metalloproteinase 2
背景信息 The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, atherosclerosis, and in cancer cell invasion and metastasis. MMP2, also known as Gelatinase A, is a type IV collagenase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme. 产品信息 格式 Purified 控制 - A431 cell lysate.
演示 Purified in 0.1M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3. 应用 应用 Detect MMP-2 using this Anti-MMP-2 Antibody, hinge region validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH conjugated synthetic peptide selected from the hinge region of human MMP-2. 表位 Hinge region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes human MMP-2. It does not cross react with MMP-1, MMP-8, MMP-9, MMP-13, and MMP-14. 品种反应性 PigHumanRatMonkey Species Reactivity Note Human and rat. Does not appear to react with NIH/3T3 cell lysate. Predicted to cross react with monkey (100%) and porcine (95%) based on sequence homology. Reactivity with other species has not been tested 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001121363
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
基因符号 - TBE-1
- CLG4
- CLG4A
- MMP-2
- MMP-II
- MONA
纯化方法 Protein G purfied UniProt编号 - P08253
UniProt汇总 FUNCTION: In addition to gelatin and collagens, it cleaves KiSS1 at a Gly-|-Leu bond.
CATALYTIC ACTIVITY: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.
COFACTOR: Binds 4 calcium ions per subunit.
Binds 2 zinc ions per subunit.
ENZYME REGULATION: Inhibited by histatin-3 1/24 (histatin-5). Ref.9
SUBUNIT STRUCTURE: Ligand for integrin alpha-V/beta-3.
SUBCELLULAR LOCATION: Secreted > extracellular space › extracellular matrixProbable.
TISSUE SPECIFICTY: Produced by normal skin fibroblasts.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3).
INVOLVEMENT IN DISEASE: Defects in MMP2 are the cause of Torg-Winchester syndrome [MIM:259600]; also called multicentric osteolysis nodulosis and arthropathy (MONA). Torg-Winchester syndrome is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
SEQUENCE SIMILARITIES: Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.产品使用声明 质量保证 Evaluated on a representative lot by Western blot on A431 cell lysate using Anti-MMP-2. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 1 year from date of receipt. 包装信息 数量 100 µg
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