merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5695,Anti-G Protein β3 subunit Antibody

  • merck millipore,默克密理博,AB5695,Anti-G Protein β3 subunit Antibody

    产品名称：Anti-G Protein β3 subunit Antibody
    产品型号：AB5695
    Anti-G Protein β3 subunit Antibody detects level of G Protein β3 subunit & has been published & validated for use in IH & WB.

    merck millipore,默克密理博,AB5695,Anti-G Protein β3 subunit Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5695,Anti-G Protein β3 subunit Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Po, B, HWB, IHCRbSerumPolyclonal Antibody
    描述
    产品目录编号 AB5695
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-G Protein β3 subunit Antibody
    Alternate Names
    • GNB3
    产品信息
    格式 Serum
    控制
    • POSITIVE CONTROL: retina. Staining for GNB3 is porcine retina is specific to cones: intense within outer segments, and in moderate in cone cell bodies and synaptic pedicles.

      NEGATIVE CONTROL: kidney.
    演示 Rabbit serum. Liquid.
    应用
    应用 Anti-G Protein β3 subunit Antibody detects level of G Protein β3 subunit & has been published & validated for use in IH & WB.
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 Western blot: 1:2,000 using ECL. The antibody reacts with proteins of ~35 kDa. Preferred gel percentage is 12%. Suggested blocking buffer is PBS containing 3% non-fat milk.

    Immunohistochemistry: 1:200 on frozen tissues sections. Suggested permeablization method is 0.1% Triton X-100. Suggested blocking buffer is PBS containing 0.2% BSA, 0.1% Tween 20, 5% normal rabbit serum and 0.1% sodium azide.

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Synthetic peptide from human GNB3.
    宿主 Rabbit
    特异性 GNB3 (G protein beta3 subunit, cone transducin beta subunit)
    品种反应性 PigBovineHuman
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_002075.2
    Entrez基因汇总 Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described.
    基因符号
    • IG
    • GNB3
    • SG
    UniProt编号
    • P16520
    UniProt汇总 FUNCTION: SwissProt: P16520 # Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
    SIZE: 340 amino acids; 37221 Da
    SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma.
    SIMILARITY: SwissProt: P16520 ## Belongs to the WD repeat G protein beta family. & Contains 7 WD repeats.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,AB5695,Anti-G Protein β3 subunit Antibody

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