merck millipore,默克密理博,AB5688,Anti-NeuroD 2 Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,AB5688,Anti-NeuroD 2 Antibody
产品名称：Anti-NeuroD 2 Antibody
产品型号：AB5688
Anti-NeuroD 2 Antibody detects level of NeuroD 2 & has been published & validated for use in IC, IH, IH(P) & WB.

merck millipore,默克密理博,AB5688,Anti-NeuroD 2 Antibody

产品介绍

merck millipore,默克密理博,AB5688,Anti-NeuroD 2 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, ICC, IHC	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5688
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-NeuroD 2 Antibody
Alternate Names	Brain bHLH Protein KW8 NDRF NeuroD-related Factor Neurogenic Differentiation Factor 2 Ndr2
背景信息	Neurogenic differentiation 2, also known as NEUROD2, is a human gene. This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates. Diseases associated with NEUROD2 are motor deficits, ataxia, and seizures. NEUROD2 expression is observed mainly in endovascular invasive cells.

产品信息
格式	Affinity Purified
控制	Cerebellum tissue
演示	Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

应用
应用	Anti-NeuroD 2 Antibody detects level of NeuroD 2 & has been published & validated for use in IC, IH, IH(P) & WB.
主要应用	Western Blotting Immunocytochemistry Immunohistochemistry
应用说明	Immunohistochemistry: 1:200-1:1,000. Immunocytochemistry: 1:500 on PC12 cells Western blot: 1:200-1:1,000 using ECL. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	Synthetic peptide, amino acids 23-39 of human NeuroD2.
宿主	Rabbit
特异性	Recognizes NeuroD 2 (NRDL, KW80), a Helix-loop-helix class of transcription factor.
品种反应性	RatHumanMouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_006160.3
Entrez基因汇总	This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates.
基因符号	MGC26304 NEUROD2 NDRF NeuroD2
UniProt编号	Q15784
UniProt汇总	FUNCTION: SwissProt: Q15784 # Appears to mediate neuronal differentiation. SIZE: 382 amino acids; 41361 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. SUBCELLULAR LOCATION: Nucleus (Probable). SIMILARITY: SwissProt: Q15784 ## Contains 1 basic helix-loop-helix (bHLH) domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. The antibody must be "rehydrated" prior to use: To rehydrate/reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 mL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 mg of precipitated antibody, the amount of buffer needed would be 50 mL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody can be stored at: 2-8°C The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. -20°C For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. -70°C For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.

储存和货运信息

存储条件

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The antibody must be "rehydrated" prior to use:
To rehydrate/reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium
sulfate/PBS solution: 10 mL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 mg of precipitated antibody, the amount of buffer needed would be 50 mL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody can be stored at:
2-8°C The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°.

-20°C For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

-70°C For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.