merck millipore,默克密理博,AB5220,Anti-Growth Associated Protein-43 (GAP-43) Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,AB5220,Anti-Growth Associated Protein-43 (GAP-43) Antibody
产品名称：Anti-Growth Associated Protein-43 (GAP-43) Antibody
产品型号：AB5220
Anti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.

merck millipore,默克密理博,AB5220,Anti-Growth Associated Protein-43 (GAP-43) Antibody

产品介绍

merck millipore,默克密理博,AB5220,Anti-Growth Associated Protein-43 (GAP-43) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
B, H, M, R, Ch	WB, ELISA, ICC, IHC	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	AB5220
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Growth Associated Protein-43 (GAP-43) Antibody
Alternate Names	Neuromodulin
背景信息	GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

产品信息
格式	Purified
控制	Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
演示	Purified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.

应用
应用	Anti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.
主要应用	Western Blotting ELISA Immunocytochemistry Immunohistochemistry
应用说明	Immunohistochemistry: 1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections. Immunocytochemistry: 1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF. Immunoprecipitation: 1:100 dilution of a previous lot was used. ELISA: 1:2,000 dilution of a previous lot was used.

生物信息
免疫原品种	Recombinant rat GAP-43 (complete sequence).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes Growth Associated Protein-43 (GAP-43).
品种反应性	BovineHumanMouseRatChicken
Species Reactivity Note	Reacts to a high degree with mouse. Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_002045.2
Entrez基因汇总	The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system.
基因符号	B-50 GAP43 PP46
纯化方法	Ammonium sulfate precipitation
UniProt编号	P17677
UniProt汇总	FUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. SIZE: 238 amino acids; 24803 Da SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity). SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes. PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on Mouse brain lysates. Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for up to 6 months at -20ºC in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

储存和货运信息

存储条件

Stable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.