merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5166-50UL,Anti-Muscarinic Acetylcholine Receptor m2 Antibody

  • merck millipore,默克密理博,AB5166-50UL,Anti-Muscarinic Acetylcholine Receptor m2 Antibody

    产品名称：Anti-Muscarinic Acetylcholine Receptor m2 Antibody
    产品型号：AB5166-50UL
    Anti-Muscarinic Acetylcholine Receptor m2 Antibody detects level of Muscarinic Acetylcholine Receptor m2 & has been published & validated for use in IH, IP & WB.

    merck millipore,默克密理博,AB5166-50UL,Anti-Muscarinic Acetylcholine Receptor m2 Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5166-50UL,Anti-Muscarinic Acetylcholine Receptor m2 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 AB5166-50UL
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Muscarinic Acetylcholine Receptor m2 Antibody
    产品信息
    格式 Affinity Purified
    控制
    • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blot using 50 ng of protein per lane. For negative control, preincubate 5-7 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
    演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
    应用
    应用 Anti-Muscarinic Acetylcholine Receptor m2 Antibody detects level of Muscarinic Acetylcholine Receptor m2 & has been published & validated for use in IH, IP & WB.
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 Western blot: 1:200 using ECL on rat brain membranes.

    Immunohistochemistry on rat brain sections.

    Immunoprecipitation (Vorobiov, 2000; Lee, 2000)

    Dilutions should be made using a carrier protein such as BSA (1-3%)

    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 GST fusion protein (Rybin, 2000) and part of i3 intercellular loop of human m2 muscarinic acetylcholine receptor (amino acids 225-356) (Accession P08172).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes a full-length m2 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far.
    品种反应性 RatHumanMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_001006632.1
    • NM_000739.2
    • NM_001006626.1
    • NM_001006627.1
    • NM_001006628.1
    • NM_001006629.1
    • NM_001006630.1
    • NM_001006631.1
    Entrez基因汇总 The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.
    基因符号
    • HM2
    • CHRM2
    • MGC120006
    • FLJ43243
    • MGC120007
    UniProt编号
    • P08172
    UniProt汇总 FUNCTION: SwissProt: P08172 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
    SIZE: 466 amino acids; 51715 Da
    SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
    SIMILARITY: SwissProt: P08172 ## Belongs to the G-protein coupled receptor 1 family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB5166-50UL,Anti-Muscarinic Acetylcholine Receptor m2 Antibody

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