merck millipore,默克密理博,07-1426,Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody

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merck millipore,默克密理博,07-1426,Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody
产品名称：Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody
产品型号：07-1426
Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr996) for use in WB & IP.

merck millipore,默克密理博,07-1426,Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody

产品介绍

merck millipore,默克密理博,07-1426,Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody

Replacement Information
Replacement Information	07-1426 is a recommended replacement for AB3847

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-1426
Replaces	AB3847
描述	Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody
Alternate Names	Vascular endothelial growth factor receptor Protein-tyrosine kinase receptor Flk-1
背景信息	The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.

产品信息
格式	Purified
控制	Huvec cell lysate
演示	Purified rabbit polyclonal IgG in buffer containing 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 µg/mL BSA, with 50% glycerol.

应用
应用	Anti-phospho-VEGFR2/Flk-1/KDR (Tyr996) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr996) for use in WB & IP.
主要应用	Western Blotting
应用说明	Immunoprecipitation: 1:50 dilution from a previous lot of this antibody was used in immunoprecipitation. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Tyr996 of human VEGF receptor-2.
宿主	Rabbit
特异性	Recognizes VEGFR-2 only when phosphorylated at tyrosine 996. This antibody cross-reacts with tyrosine-phosphorylated PDGF receptor but does not cross-react with other tyrosine-phosphorylated proteins.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human. Predicted to react with mouse due to 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_002253.1
基因符号	EC 2.7.10.1 KDR VEGFR-2 VEGFR2 CD309 FLK1 VEGFR
修改	Phosphorylation
纯化方法	Protein A and Affinity Purified
UniProt编号	P35968
UniProt汇总	FUNCTION: Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 1 protein kinase domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on pervanadate treated Huvec lysates. Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected phosphorylated VEGFR2 on 10 μg of pervanadate treated Huvec lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt, do not aliquot. Handling Recommendations: Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt, do not aliquot.
Handling Recommendations: Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.