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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3609 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Smac/Diablo Antibody 背景信息 The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues. 产品信息 格式 Purified 演示 Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide. 应用 应用 This Anti-Smac/Diablo Antibody is validated for use in WB for the detection of Smac/Diablo. 主要应用 - Western Blotting
应用说明 Western blot: 1:500 to 1:1000
Human heart tissue lysate can be used as a positive control.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Peptide corresponding to aa 225-239 (EERAESEQEAYLRED) of human Smac/DIABLO. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Detects Smac/DIABLO in an approximately 25 kDa band by western blot. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_138930.2
- NM_019887.3
- NM_138929.2
Entrez基因汇总 This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. 基因符号 - 0610041G12Rik
- DIABLO
- diablo
- SMAC
- FLJ10537
- DIABLO-S
- FLJ25049
- SMAC3
UniProt编号 - Q9NR28
UniProt汇总 FUNCTION: SwissProt: Q9NR28 # Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
SIZE: 239 amino acids; 27131 Da
SUBUNIT: Interacts with NGFRAP1/BEX3 (By similarity). Homodimer. Interacts with BIRC2, BIRC3, BIRC4/XIAP and BIRC7.
SUBCELLULAR LOCATION: Mitochondrion. Note=But released into the cytosol when cells undergo apoptosis.
TISSUE SPECIFICITY: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
DOMAIN:SwissProt: Q9NR28 The mature N-terminus mediates interaction with BIRC4/XIAP.
SIMILARITY:产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 4°C for up to 6 months. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,14-515,MAP Kinase 1/Erk1 Protein, unactive, 50 µg
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