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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, ELISA, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3046 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Uncoupling Protein 3 Antibody Alternate Names - UCP3
背景信息 Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein. It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long. UCP3 has preferential expression in muscle and it is unaffected by cold acclimation. 产品信息 格式 Affinity Purified 控制 - Muscle tissue, Blocking peptide for UCP3 is available (Catalogue No. AG769).
演示 Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA. 应用 应用 Anti-Uncoupling Protein 3 Antibody is an antibody against Uncoupling Protein 3 for use in ELISA, IH & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 Western Blotting: 1-10 μg/mL using Chemiluminescence technique.
Immunohistochemistry: 2-20 μg/mL on formaldehyde fixed tissue.
ELISA: (1:10,000-1:100,000 using 50-100 ng UCP32 control peptide/well).
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 14 amino acid peptide sequence (designated UCP32) mapping near the C-terminus of human UCP3 (Boss et al. 1997). The UCP32 peptide has no significant homology with UCP1 or UCP2. 宿主 Rabbit 特异性 Uncoupling Protein 3 (UCP3), human. It is expected that the antibody will recognize both the long and short forms (30-33 kDa & 24-27 kDa) however splice variants are expected and precise reactivity profile has yet to be determined. Human UCP3 long form (UCP3L) is a 312 aa mitochondrial uncoupling protein (Boss et al. 1997; Vidal-Puig et al. 1997). It is only 57% and 73% homologous with human UCP1 and UCP2 respectively. Like other UCPs, UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and is only 275 aa long (Vidal-Puig et al. 1997). UCP3 has preferential expression in muscle and it is unaffected by cold acclimation. 品种反应性 RatHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_022803.1
- NM_003356.2
Entrez基因汇总 Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene has tissue-specific transcription initiation with other transcription initiation sites upstream of SM-1 (major skeletal muscle site). Chromosomal order is 5'-UCP3-UCP2-3'. Two splice variants have been found for this gene. 基因符号 - SLC25A9
- UCP3
纯化方法 Immunoaffinity purified UniProt编号 - P55916
UniProt汇总 FUNCTION: SwissProt: P55916 # UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance.
SIZE: 312 amino acids; 34216 Da
SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles.
DISEASE:SwissProt: P55916 # Defects in UCP3 may be involved in severe obesity [MIM:601665].
SIMILARITY: SwissProt: P55916 ## Belongs to the mitochondrial carrier family. & Contains 3 Solcar repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 50 µg
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