merck millipore,默克密理博,CYT420,ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA

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merck millipore,默克密理博,CYT420,ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA
产品名称：ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA
产品型号：CYT420
This ChemiKine Pigment Epithelium Derived Factor, Sandwich ELISA is used to measure & quantify PEDF levels in Neuroscience research.

merck millipore,默克密理博,CYT420,ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA

产品介绍

merck millipore,默克密理博,CYT420,ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
PEDF	H	ELISA	Colorimetric

描述
产品目录编号	CYT420
品牌系列	Chemicon®
商名	Chemicon ChemiKine
描述	ChemiKine™ Pigment Epithelium Derived Factor, Sandwich ELISA
概述	Introduction Pigment Epithelium-Derived Factor (PEDF) was first identified as a factor secreted by primary retinal pigment epithelial (RPE) cells that promotes the survival of neurons in vitro and in vivo. Molecular characterization of PEDF shows it to be a 50 kDa member of the serpin family of protease inhibitors1. A second mode of action of PEDF was identified with the demonstration that PEDF induces apoptosis of microvascular endothelial cells, a property that maintains the avascular nature of the retina2. As a number of ophthalmologic disorders are characterized by dysregulation of the retinal innervation and microvasculature, PEDF serves as an important regulator of human eye disease3. In addition, PEDF appears to have multifunctional antitumor activity in a neuroblastoma model, as Schwann cell-derived PEDF induces a differentiated, less malignant phenotype in neuroblastoma cells, promotes further growth and survival of Schwann cells, and inhibits angiogenesis4. ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin. Test Principle With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects the captured PEDF. Peroxidase-labeled streptavidin detects the biotinylated mouse monoclonal antibody. After addition of the substrate and stop solution the amount of PEDF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and PEDF concentration: i.e., the higher the OD the higher the PEDF concentration in the sample.
Alternate Names	PEDF

产品信息
部件	Stop Solution (Part No. 60193): One 12 mL bottle of an HCl solution (Ready to Use). PEDF ELISA Plate: (Part No. 61597) One 96-Well Immunoplate Pre-coated with Mouse anti-Human PEDF Monoclonal Antibody, sealed in a foil pouch. Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate. Assay Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use). PEDF Standard (Human): (Part No. 61600) One vial containing 250 ng recombinant full length human PEDF (Lyophilized). Biotinylated Mouse anti-Human PEDF Monoclonal Antibody: (Part No. 61598) One 50 μL vial. Streptavidin, HRP conjugated: (Part No. 61599) One 50 μL vial. TMB/E Solution: (Part No. 60096) One 10 mL bottle of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
检测方法	Colorimetric

产品信息

部件

Stop Solution (Part No. 60193): One 12 mL bottle of an HCl solution (Ready to Use).
PEDF ELISA Plate: (Part No. 61597) One 96-Well Immunoplate Pre-coated with Mouse anti-Human PEDF Monoclonal Antibody, sealed in a foil pouch.
Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.
Assay Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).
PEDF Standard (Human): (Part No. 61600) One vial containing 250 ng recombinant full length human PEDF (Lyophilized).
Biotinylated Mouse anti-Human PEDF Monoclonal Antibody: (Part No. 61598) One 50 μL vial.
Streptavidin, HRP conjugated: (Part No. 61599) One 50 μL vial.
TMB/E Solution: (Part No. 60096) One 10 mL bottle of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.

检测方法

Colorimetric

应用
应用	This ChemiKine Pigment Epithelium Derived Factor, Sandwich ELISA is used to measure & quantify PEDF levels in Neuroscience research.
主要应用	ELISA
应用说明	ChemiKine™ PEDF kit is designed to measure the amount of PEDF in cell culture supernatants, tissue homogenates and biological fluid (vitreous, serum, plasma, and serum-free) samples of human origin. There are enough reagents included in this kit for one 96-well immunoassay plate. Running duplicate wells for samples and standards is recommended. For Research Use Only. Not for use in diagnostic procedures.

生物信息
宿主	Mouse
品种反应性	Human
抗体类型	Monoclonal Antibody
可用的分析物	PEDF
Entrez基因编号	NM_002615.4
基因符号	EPC-1 SERPINF1 Serpin-F1 PEDF PIG35
UniProt编号	P36955
UniProt汇总	FUNCTION: SwissProt: P36955 # Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. SIZE: 418 amino acids; 46342 Da SUBCELLULAR LOCATION: Secreted. Melanosome. Note=Enriched in stage I melanosomes. TISSUE SPECIFICITY: Retinal pigment epithelial cells and blood plasma. DEVELOPMENTAL STAGE: Expressed in quiescent cells. DOMAIN: SwissProt: P36955 The N-terminal (AA 44-121) exhibits neurite outgrowth- inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. PTM: The N-terminus is blocked. SIMILARITY: Belongs to the serpin family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain the unopened kit at 2-8°C until expiration date indicated on the label. After opening the kit maintain each component at 2-8°C until expiration date indicated on the label. After reconstitution, maintain the PEDF Standard at -20ºC for up to 30 days. The standard may be frozen and thawed three times without loss of immunoreactivity. Do not store reconstituted standard at 4ºC, as loss of immunoreactivity will result. Precautions · Wash Buffer and Assay Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly. · The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.

储存和货运信息

存储条件

Maintain the unopened kit at 2-8°C until expiration date indicated on the label. After opening the kit maintain each component at 2-8°C until expiration date indicated on the label. After reconstitution, maintain the PEDF Standard at -20ºC for up to 30 days. The standard may be frozen and thawed three times without loss of immunoreactivity. Do not store reconstituted standard at 4ºC, as loss of immunoreactivity will result.

Precautions

· Wash Buffer and Assay Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

· The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.