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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ELISA, EMSA Rb Purified Polyclonal Antibody 描述 产品目录编号 AB3006 Replaces 04-218 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-CREB Antibody Alternate Names - cAMP-response Element-binding Protein-1
- CREB-1
背景信息 CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2. 产品信息 格式 Purified 控制 - Forskolin-treated PC12 cell lysate
演示 Purified immunoglobulin. Liquid (sterile filtered) in 0.02M Potassium Phosphate buffer containing 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide. 应用 应用 Use Anti-CREB Antibody (Rabbit Polyclonal Antibody) validated in ELISA, EMSA, IP, WB to detect CREB also known as CREB-1, cAMP-response Element-binding Protein-1. 主要应用 - Western Blotting
- ELISA
- Electrophoretic Mobility Shift Assay
应用说明 Immunoblot
Immunoprecipitation
ELISA
Supershift assay: 0.5-1.0 μL per assay.
Optimal dilutions must be determined by the end user.生物信息 免疫原品种 CREB-1 (p43) peptide corresponding to the N-terminal (aa 5-45) of human protein conjugated to KLH. 宿主 Rabbit 特异性 Human cyclic AMP responsive element binding protein-1 (CREB-1, p43). By Western blot it shows a major band at 46 kDa when assayed against a lysate of 1 x 106 Raji B cells. Minor bands at 75 kDa and 43 kDa may be noted. Partial reactivity to CREM-1 protein. May react non-specifically with other proteins. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_134442.2
- NM_004379.2
Entrez基因汇总 This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. 基因符号 - CREB
- CREB1
- MGC9284
UniProt编号 - P16220
UniProt汇总 FUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.
SIZE: 341 amino acids; 36688 Da
SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.
SUBCELLULAR LOCATION: Nucleus.
PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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