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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2964 Replaces AB19051 描述 Anti-MMP-12 Antibody, hinge region Alternate Names - matrix metallopeptidase 12
- Macrophage elastase
- Matrix metalloproteinase-12
- macrophage metalloelastase
背景信息 Matrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced. 产品信息 格式 Affinity Purified 控制 - THP-1/PMA-conditioned media lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-MMP-12 Antibody, hinge region detects level of MMP-12 & has been published & validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human MMP-12. 表位 Hinge Region 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes the hinge region of MMP-12. 品种反应性 Human Species Reactivity Note Demonstrated to react with human.
Other homologies: Chimpanzee and rhesus macaque (95% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002417
Entrez基因汇总 Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. 基因符号 - MMP 12
- MMP12
- MMP-12
- MME
- ME
- HME
纯化方法 Affinity Purified UniProt编号 - P39900
UniProt汇总 FUNCTION: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 470 amino acids; 54002 Da
TISSUE SPECIFICITY: Found in alveolar macrophages but not in peripheral blood monocytes.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.产品使用声明 质量保证 Evaluated by Western Blot in THP-1/PMA-conditioned media lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MMP-12 in 10 µg of THP-1/PMA-conditioned media lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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