- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB2953 描述 Anti-ATG3 Antibody Alternate Names - Protein PC3-96
- ATG3 autophagy related 3 homolog (S. cerevisiae)
- APG3 autophagy 3-like (S. cerevisiae)
- autophagy-related protein 3
- autophagy Apg3p/Aut1p-like
- APG3 autophagy 3-like
背景信息 ATG3 is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE). This compound is necessary for autophagy, which is a process in which a cell is degraded by its own lysosomal process. 产品信息 格式 Affinity Purified 控制 - HL-60 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ATG3 Antibody detects level of ATG3 & has been published & validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human ATG3. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Demonstrated to react with human.
Other homologies: Ape, rhesus macaque, equine, porcine, and bovine (99% sequence homology). Mouse (98% sequence homology). Rat (97% sequence homology).抗体类型 Polyclonal Antibody Entrez基因编号 - NP_071933
Entrez基因汇总 Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM]. 基因符号 - APG3-like
- ATG3
- PC3-96
- APG3-LIKE
- APG3
- hApg3
- APG3L
纯化方法 Affinity Purified UniProt编号 - Q9NT62
UniProt汇总 FUNCTION: E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
SUBUNIT STRUCTURE: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm
TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
PTM: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy (By similarity).
SEQUENCE SIMILARITIES: Belongs to the ATG3 family.
SEQUENCE CAUTION: The sequence BAB15237.1 differs from that shown. Reason: Erroneous initiation.
Isoform 2: The sequence AAH02830.1 differs from that shown. Reason: Frameshift at position 311.产品使用声明 质量保证 Evaluated by Western Blot in HL-60 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected ATG3 in 10 µg of HL-60 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,04-419,Anti-phospho-Rsk1 (Thr359/Ser363) Antibody, rabbit monoclonal
下一件merck millipore产品：merck millipore,默克密理博,IP02,Protein A Agarose Suspension
相关产品
merck millipore,默克密理博,SF-3005,CRK; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,539251,Protein A, Alkaline Phosphatase Conjugate
merck millipore,默克密理博,361524,GSK-3β, His•Tag, Human, Recombinant, E. coli
merck millipore,默克密理博,04-419,Anti-phospho-Rsk1 (Thr359/Ser363) Antibody, rabbit monoclonal
merck millipore,默克密理博,IP02,Protein A Agarose Suspension
merck millipore,默克密理博,512533,MKLP-2 Inhibitor, Paprotrain - Calbiochem
merck millipore,默克密理博,341597,FGF-II, Mouse, Recombinant, E. coli
merck millipore,默克密理博,FCMAB135F,Milli-Mark Anti-BMP-7-FITC Antibody, clone 2A10