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品种反应性 主要应用 宿主 格式 抗体类型 M, R ICC, WB, IHC Gp Serum Polyclonal Antibody 描述 产品目录编号 AB2253 Replaces AB5910 描述 Anti-Doublecortin Antibody Alternate Names - lissencephaly, X-linked (doublecortin)
- doublecortex
- lissencephaly
背景信息 Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express, a marker for mature neurons. Due to the nearly exclusive expression of DCX in developing neurons, this protein has been used increasingly as a marker for neurogenesis. Indeed, the levels of DCX expression increase in response to exercise, which occurs in parallel with increased BrdU labelling, currently a "gold standard" in measuring neurogenesis. 产品信息 格式 Serum 控制 - Rat Brain lysate.
演示 GST-Depleted Sera with 0.05% NaN3 . 应用 应用 This Anti-Doublecortin Antibody is validated for use in WB, IH, IC for the detection of Doublecortin. 主要应用 - Immunocytochemistry
- Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
1:1,000 – 1:15,000 on free floating brain sections. Optimal dilutions need to be determined by the end user.
Immunocytochemistry:
Tested on mouse primary neurons. Optimal dilutions must be determined by end user.
生物信息 免疫原品种 Recombinant Peptide. 表位 C-Terminus 宿主 Guinea Pig 特异性 Cat. # AB2253 recognizes the C-Terminus of Doublecortin. 品种反应性 MouseRat Species Reactivity Note Mouse and Rat. Expected to react with human based on sequence homology. Reactivity with other species has not been determined. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000555.2
Entrez基因汇总 In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene. [provided by RefSeq]
基因符号 - lissencephalin-X
- DBCN
- DC
- Doublin
- LISX
- Lis-X
- Lissencephalin-X
- OTTHUMP00000062892
- SCLH
- XLIS
- doublecortex
- doublecortin
- doublin
纯化方法 GST Depletion UniProt编号 - O43602
UniProt汇总 FUNCTION: Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
SUBUNIT STRUCTURE: Interacts with tubulin.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
DISEASE: Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'. Ref.1 Ref.2 Ref.9 Ref.10 Ref.18 Ref.21
Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal. Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19
A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
SEQUENCE SIMILARITY: Contains 2 doublecortin domains.
产品使用声明 质量保证 Routinely evaluated by western blot in rat brain lysate.
Western Blot:
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. 包装信息 数量 50 µL
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