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品种反应性 主要应用 宿主 格式 抗体类型 Chp, R, H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 ABS22 描述 Anti-PDE4D Antibody Alternate Names - phosphodiesterase 4D, cAMP-specific (dunce)
- phosphodiesterase E3 dunce homolog
- phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
- dunce-like phosphodiesterase E3
- cAMP-specific phosphodiesterase 4D
- phosphodiesterase 4D, cAMP-specific
- cAMP-specific phosphodiesterase PDE4D6
- cAMP-specific 3',5'-cyclic phosphodiesterase 4D
- phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3)
背景信息 Phosphodiesterase 4D (PDE4D) is a member of the PDE cyclic nucleotides. PDE4D is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D may be associated with analgesic and anti-inflammatory effects. It has also been linked to the risk of stroke, specifically common polygenic stroke. 产品信息 格式 Purified 控制 - Rat brain tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-PDE4D Antibody is an antibody against PDE4D for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the C-terminus of human PDE4D. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes PDE4D at the C-terminus. 品种反应性 ChimpanzeeRatHuman Species Reactivity Note Demonstrated to react with Human and Rat. Predicted to react with Chimpanzee based on 100% sequence homology.
Expected to react with Mouse based on 90% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001098101
Entrez基因汇总 This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins. 基因符号 - HSPDE4D
- PDE4DN2
- DPDE3
- PDE43
- PDE4D
- STRK1
纯化方法 Protein A UniProt编号 - Q08499
UniProt汇总 FUNCTION: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
CATALYTIC ACTIVITY: Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions. Ref.26 Ref.27
ENZYME REGULATION: Inhibited by rolipram. Activated by phosphatidic acid.
PATHWAY: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
SUBUNIT STRUCTURE: Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 2 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity). Isoform 6, isoform 7 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane (By similarity). Cytoplasm › cytoskeleton (By similarity). Cytoplasm › cytoskeleton › centrosome (By similarity). Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity).
TISSUE SPECIFICTY: Widespread; most abundant in skeletal muscle. Isoform 8 is detected in brain. Isoform 9 is detected in brain, placenta, lung and kidney. Isoform 11 is detected in heart and skeletal muscle.
PTM: Isoform 2 and isoform 11 are activated by phosphorylation (in vitro), but not isoform 8. Isoform 7 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
INVOLVEMENT IN DISEASE: Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. Ref.16 states that association with stroke has to be considered with caution.
SEQUENCE SIMLARITIES: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.产品使用声明 质量保证 Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PDE4D on 10 µg of rat brain tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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