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merck millipore,默克密理博,AB1987,Anti-Neurofilament M (145 kDa) Antibody, CT
产品名称：Anti-Neurofilament M (145 kDa) Antibody, CT
产品型号：AB1987
Anti-Neurofilament M (145 kDa) Antibody, C-terminus detects level of Neurofilament M (145 kDa) & has been published & validated for use in IC, IH, IH(P) & WB.
merck millipore,默克密理博,AB1987,Anti-Neurofilament M (145 kDa) Antibody, CT
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Rp, B, Ch, H, M, Po, R, Qu, Rb WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1987 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Neurofilament M (145 kDa) Antibody, CT 背景信息 Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. 产品信息 格式 Serum 控制 - Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve
演示 Neat rabbit antiserum containing no preservative. 应用 应用 Anti-Neurofilament M (145 kDa) Antibody, C-terminus detects level of Neurofilament M (145 kDa) & has been published & validated for use in IC, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
A 1:200 dilution of a previous lot was used in Immunohistochemistry.
Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Immunohistochemistry(paraffin):
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Recombinant fusion protein containing the C-terminal 168 amino acids of rat NF-M 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Strong and specific staining for NF-M (145 kDa) 品种反应性 ReptileBovineChickenHumanMousePigRatQuailRabbit Species Reactivity Note Rat, human, mouse, pig, cow(bovine), chicken, quail, alligator and tortoise. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001105541.1
基因符号 - NFM
- NEFM
- NF-M
- NEF3
纯化方法 Unpurified UniProt编号 - P07197
UniProt汇总 FUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
SIZE: 916 amino acids; 102448 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.产品使用声明 质量保证 Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 50 µL
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