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品种反应性 主要应用 宿主 格式 抗体类型 Sh, B, Ca, Ch, Gt, Ht, Eq, H, M, Po, R WB, ELISA, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1920 Replaces AB1948 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Integrin α3 Antibody Alternate Names - CD49c
背景信息 Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. Both of the subunits contribute to the binding of the ligand. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys. 产品信息 格式 Serum 控制 - Human fetal foreskin fibroblasts (HFFF), neuroblastomas
演示 Rabbit polyclonal IgG antiserum containing 0.05% sodium azide. 应用 应用 Detect Integrin α3 using this Anti-Integrin α3 Antibody validated for use in ELISA, IF, IH, IP, RIA & WB. 主要应用 - Western Blotting
- ELISA
- Immunohistochemistry
应用说明 ELISA/RIA:
A 1:1,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay/Radioimmunoassay.
Immunoprecipitation:
5 µL of a previous lot precipitated alpha3/beta1 from 5 x 106 cells (Sherry, D.M., 2001).
Immunohistochemistry/immunofluorescence:
1:500 dilution of a previous lot was used, suggest use on acetone-fixed tissue.
Western Blot Analysis:
1:500 dilution of a previous lot was used.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 A synthetic peptide (CRIQPSETERLTDDY) derived from the COOH terminal sequence located in the cytoplasmic domain of the human alpha3 integrin subunit. 表位 Cytoplasmic domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Integrin alpha3. Specificity determined by immunoprecipitation from a detergent extract of 35S-methionine labeled neuroblastoma cells. No cross-reactivity to alpha1, alpha2, alpha4, alpha5, alpha6, or alphaV integrins. 品种反应性 SheepBovineCanineChickenGoatHamsterHorseHumanMousePigRat Species Reactivity Note Human, mouse, rat, hamster, bovine, equine, sheep, porcine, canine, chick, and goat. Reactivity with other species has not been determined. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002204.1
Entrez基因汇总 ITGA3 encodes the integrin alpha 3 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular-matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 ('VLA-3'), very common antigen 2 ('VCA-2'), extracellular matrix receptor 1 ('ECMR1'), and galactoprotein b3 ('GAPB3'). 基因符号 - CD49C
- ITGA3
- GAP-B3
- CD49c
- FLJ34704
- MSK18
- VLA3a
- VL3A
- VCA-2
- FLJ34631
- FRP-2
- GAPB3
纯化方法 Unpurified UniProt编号 - P26006
UniProt汇总 FUNCTION: SwissProt: P26006 # Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
SIZE: 1066 amino acids; 118698 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-3 associates with beta-1. Interacts with HPS5. />SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
PTM: Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12- myristate 13-acetate stimulation.
SIMILARITY: SwissProt: P26006 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.产品使用声明 质量保证 Routinely evaluated by Western Blot on A431 lysates.
Western blot: 1:500 dilution of this lot detected INTEGRIN ALPHA 3 on 10 μg of A431 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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