merck millipore,默克密理博,17-608,ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set

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Merck Millipore 默克密理愽

品种反应性	主要应用
M, H	ChIP, WB

描述
产品目录编号	17-608
品牌系列	Upstate
商名	Upstate ChIPAb+
描述	ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ HDAC1 set includes the HDAC1 antibody, the negative control antibody supernatant (mouse IgG), and qPCR primers flanking an Sp1 binding site in human p21 (WAF1/CIP1/CDKN1A) promoter, amplifying a 105 base pair PCR product. The HDAC1 and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1 associated chromatin.

产品信息
格式	Culture Supernatant
控制	Included negative control antibody supernatant mouse IgG and control primers specific for human p21 (WAF1/CIP1/CDKN1A) promoter.
演示	Anti-HDAC1 (mouse monoclonal IgG1,supernatant). One vial containing 40 μL of culture supernatant with 0.05% sodium azide. Store at -20°C. Negative ChIP Control Supernatant. One vial containing 40 uL of mouse IgG containing supernatant with 0.05% sodium azide. Store at -20°C. ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C. P21 Forward: CCC ACA GCA GAG GAG AAA GAA P21 Reverse: CTG GAA ATC TCT GCC CAG ACA

应用
应用	This ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Western Blotting
应用说明	Chromatin Immunoprecipitation Analysis: Sonicated chromatin prepared from untreated or actinomycin D treated (30 ng/mL, 6 hrs.) U2OS cells (3 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 4 μL of Mouse Anti-HDAC1 and the Magna ChIP™ G (Cat. # 17-611) Kit. Immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers p21 flanking the human p21 promoter that contains an Sp1 binding site (Please see figures). Fold Decrease is a ratio of normalized mean IP quantities extracted from standard curves derived from inputs of each chromatin sample. As previously published, HDAC1 association with this region of the p21 promoter decreases upon actinomycin D treatment. Western Blot Analysis: 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC1 (1:2500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

生物信息
免疫原品种	The HDAC1 antibody is made against a peptide corresponding to amino acids 467-482 of mouse HDAC1.
宿主	Mouse
特异性	HDAC1
同种型	IgG1
品种反应性	MouseHuman
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_032254
基因符号	RPD3L1 HD1 HDAC1
UniProt编号	Q13547

产品使用声明
质量保证	Routinely evaluated by chromatin immunoprecipitation on U2OS cells and immunoblot on 3T3/A31 nuclear lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt

包装信息
数量	10 assays
包装	10 assays per kit, ~4μL per chromatin immunoprecipitation

merck millipore,默克密理博,17-608,ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set

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