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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Sh WB, ELISA Rb Serum Polyclonal Antibody 描述 产品目录编号 AB1839 Replaces 04-1095 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Interleukin-6 Antibody Alternate Names - IL-6
产品信息 格式 Serum 演示 Rabbit serum. Liquid containing 0.1% sodium azide. 应用 应用 Anti-Interleukin-6 Antibody is an antibody against Interleukin-6 for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Detecting antibody in ELISA, using monoclonal anti-ovine IL-6 as capture antibody. Recommended dilution factor 1:500. See suggested protocol.
Western Blot
Optimal working dilutions must be determined by the end user.
Suggested ELISA Protocol For Ovine IL-6
1. Coat high capacity microtitre wells (e.g. Nunc maxisorp) with monoclonal anti-IL-6 antibody (Chemicon Catalog Number MAB1004) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4ºC.
2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).
3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.
4. Dilute rabbit anti-IL-6 (Chemicon Catalog Number AB1839) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.
5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.
6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulphuric acid.
Notes:
The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.
Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.
Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.生物信息 免疫原品种 Recombinant ovine IL-6 宿主 Rabbit 特异性 Reacts with ovine IL-6. No reactivity detected to recombinant ovine IL-1beta, IL-8, MCP or TNFalpha. 品种反应性 Sheep 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000600.2
基因符号 - IFNB2
- IL6
- HSF
- BSF-2
- HGF
- IL-6
- BSF2
- CDF
UniProt编号 - P05231
UniProt汇总 FUNCTION: SwissProt: P05231 # IL-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
SIZE: 212 amino acids; 23718 Da
SUBCELLULAR LOCATION: Secreted.
PTM: N- and O-glycosylated.
DISEASE: SwissProt: P05231 # Genetic variations in IL6 are associated with susceptibility to systemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenile rheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despite medical treatment, many children still experience early joint destruction, necessitating surgical replacement. & At least 1 polymorphism in the IL6 gene renders HIV- infected men susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma of spindle cells mixed with angiomatous tissue. A relatively rare malignant skin tumor that results in multifocal purplish coloured papules or plaques that eventually form nodules. Most commonly seen in patients who suffer from AIDS.
SIMILARITY: SwissProt: P05231 ## Belongs to the IL-6 superfamily.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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