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merck millipore,默克密理博,04-586,Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1
产品名称：Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1
产品型号：04-586
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Cytokeratin 18 Antibody, Rabbit, clone E43 More>>
merck millipore,默克密理博,04-586,Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M FC, WB, ICC Rb Purified Monoclonal Antibody 描述 产品目录编号 04-586 品牌系列 Upstate
商名 - Upstate
描述 Anti-Cytokeratin 18 Antibody, rabbit monoclonal, clone E431-1 背景信息 Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4). 产品信息 格式 Purified 控制 - Positive control: A431 cell lysate.
演示 Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Cytokeratin 18 Antibody, Rabbit, clone E431-1 is validated for use in WB, IC, IH(P), FC for the detection of Cytokeratin 18. 主要应用 - Flow Cytometry
- Western Blotting
- Immunocytochemistry
应用说明 Western Blot: 1:10,000
Immunohistochemistry: 1:100-1:250 (Paraffin-embedded colon carcinoma)
Immunocytochemistry: 1:100
Flow Cytometry: 1:20
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 A synthetic peptide corresponding to residues of human Cytokeratin 18. 克隆 E431-1 宿主 Rabbit 特异性 Recognizes Human, Mouse, Rat Cytokeratin 18. 品种反应性 RatHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_199187.1
- NM_000224.2
Entrez基因汇总 KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. 基因符号 - CK-18
- KRT18
- K18
- CYK18
- Keratin-18
- Cytokeratin-18
纯化方法 Protein A purfied UniProt编号 - P05783
UniProt汇总 FUNCTION: SwissProt: P05783 # Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
SIZE: 430 amino acids; 48058 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-18 associates with keratin-8. Interacts with the thrombin- antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD.
SUBCELLULAR LOCATION: Cytoplasm, perinuclear region.
TISSUE SPECIFICITY: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
PTM: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. & Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.DISEASE:SwissProt: P05783 # Defects in KRT18 are a cause of cryptogenic cirrhosis [MIM:215600].
SIMILARITY: SwissProt: P05783 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20 °C. Stable for 12 months from date of receipt. 包装信息 数量 100 µL
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