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品种反应性 主要应用 宿主 格式 抗体类型 Ma, H WB, ICC, IHC Gt Serum Polyclonal Antibody 描述 产品目录编号 AB1620 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Vimentin Antibody 产品信息 格式 Serum 控制 - Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter
演示 Neat goat Serum. Liquid containing 0.1% sodium azide. 应用 应用 Anti-Vimentin Antibody detects level of Vimentin & has been published & validated for use in IC, IH, IH(P) & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry: 1:20-1:40. Shows reactivity on frozen tissue sections as well as formalin-fixed paraffin embedded tissue by immunoperoxidase and immunofluorescent assays.
Immunocytochemistry
Western blot
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Vimentin purified from cultured human foreskin fibroblasts. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Goat 特异性 Recognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments. Shows wide cross-reactivity among mammalian species using various immunolabeling techniques. 品种反应性 MammalsHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003380.2
Entrez基因汇总 Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM] 基因符号 - vimentin
- VIM
- FLJ36605
- Vimentin.
纯化方法 Unpurified UniProt编号 - P08670
UniProt汇总 FUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
SIZE: 466 amino acids; 53652 Da
SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 500 µL
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