- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB15502 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Calcium Transporter 2 Antibody Alternate Names - CaT-2, ECAC1
- CaT-2
背景信息 CaT2 (rat 723 aa; human 729 aa, chromosome 7q35) is also known as ECAC1 or TRPV5) is ~75% homologous with CAT1. In contrast to CaT-1, CaT-2 is exclusively expressed in kidney and absent in intestine, brain, heart, liver, lung, skeletal muscle, spleen, thymus, testis, adrenal gland. It co-localizes with calbindin D28K and Na+ channel exchanger 1 (NCX1). CaT-2 mediates saturable apical Ca++ uptake by the cells of distal convoluted tubule and connecting segment of nephron where active re-absorption of Ca++ takes place via transcellular route. CaT-2 has moderate abilities to transport Sr++ and Ba++. A strong inhibition of CaT-2 mediated Ca++ transport by Cd++ leads to hypercalciurea and renal stone formation. 产品信息 格式 Affinity Purified 演示 Liquid in PBS with 0.1% BSA and 0.05% sodium azide. 应用 应用 Anti-Calcium Transporter 2 Antibody is an antibody against Calcium Transporter 2 for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1-10 µg/mL using ECL. However, posttranslational modification may alter its size depending upon the species and cell type.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide near the C-terminus of rat CaT-2. 宿主 Rabbit 特异性 Calcium transporter 2 (CaT-2, ECAC1). The immunogen sequence has no significant homology with CaT-1 or other proteins. 品种反应性 Rat Species Reactivity Note Other species have not yet been tested. The immunogen sequence is 90% conserved in rabbit, 80% in human and 85% in mouse Cat-2/ECAC1-1. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_019841.4
Entrez基因汇总 This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. 基因符号 - CAT2
- TRPV5
- ECaC1
- ECaC
- ECAC1
- CaT2
- TrpV5
- OTRPC3
纯化方法 Immunoaffinity purified UniProt编号 - Q9NQA5
UniProt汇总 FUNCTION: SwissProt: Q9NQA5 # Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)- dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).
SIZE: 729 amino acids; 82551 Da
SUBUNIT: Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY, which results in its inactivation (By similarity).
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein (By similarity). Note=Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells (By similarity).TISSUE SPECIFICITY: Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum.
PTM: Glycosylated (By similarity).
SIMILARITY: SwissProt: Q9NQA5 ## Belongs to the transient receptor family. TrpV subfamily. & Contains 5 ANK repeats.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,345762,GDF-8/Myostatin, Human, Mouse, Rat, Recombinant
下一件merck millipore产品：merck millipore,默克密理博,07-1317,Anti-phospho-Leptin receptor (Tyr1077) Antibody
相关产品
merck millipore,默克密理博,ST1630,Anti-FGF8 Mouse mAb (2A10)
merck millipore,默克密理博,04-1029,Anti-SMAD2 Antibody, clone EP567Y, rabbit monoclonal
merck millipore,默克密理博,ST1535,Anti-NQO1 Rabbit pAb
merck millipore,默克密理博,07-581,Anti-phospho-IRF-3 (Ser398) Antibody
merck millipore,默克密理博,345762,GDF-8/Myostatin, Human, Mouse, Rat, Recombinant
merck millipore,默克密理博,07-1317,Anti-phospho-Leptin receptor (Tyr1077) Antibody
merck millipore,默克密理博,475893,MES, Free Acid, ULTROL Grade - CAS 4432-31-9 - Calbiochem
merck millipore,默克密理博,565765,γ₄₀-Secretase Inhibitor I - Calbiochem
merck millipore,默克密理博,580563,TCPOBOP - CAS 76150-91-9 - Calbiochem
merck millipore,默克密理博,MAB8532,Anti-Herpes Virus 6 Antibody, induced early nuclear protien, clone C5