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merck millipore,默克密理博,AB144P-200UL,Anti-Choline Acetyltransferase Antibody
产品名称：Anti-Choline Acetyltransferase Antibody
产品型号：AB144P-200UL
This Anti-Choline Acetyltransferase Antibody is validated for use in IC, IH, WB for the detection of Choline Acetyltransferase.
merck millipore,默克密理博,AB144P-200UL,Anti-Choline Acetyltransferase Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Op, M, R, H, Ch, Av, Gp WB, ICC, IHC Gt Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB144P-200UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Choline Acetyltransferase Antibody Alternate Names - choline acetyltransferase
- Choline acetylase
- acetyl CoA:choline O-acetyltransferase
背景信息 Acetylcholine (ACh) is a common neuro-transmitter for motoneurons, preganglionic autonomic neurons, postganglionic para-sympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker. 产品信息 格式 Affinity Purified 控制 - Rat hypothalamus tissue.
演示 Purified goat polyclonal in buffer containing PBS containing 5 mg/mL BSA and 0.2% sodium azide. 应用 应用 This Anti-Choline Acetyltransferase Antibody is validated for use in IC, IH, WB for the detection of Choline Acetyltransferase. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry:
A previous lot of this antibody was used in immunohistochemistry at 1:100 dilution. See attached protocol.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Human placental enzyme. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Goat 特异性 Choline acetyltransferase found in cholinergic neurons in brain and central nervous systems. 品种反应性 OpossumMouseRatHumanChickenAvianGuinea Pig Species Reactivity Note Rat, mouse, monkey, guinea pig, chicken, opossum, avian and human (western blot). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_065574
Entrez基因汇总 This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq].
Transcript Variant: This variant (M) contains alternate 5' exon M, uses an upstream start codon, and encodes isoform 2.基因符号 - CMS1A2
- CHAT
- ChAT
- CHOACTase
- CMS1A
纯化方法 Immunoaffinity purified UniProt编号 - P28329
UniProt汇总 FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.产品使用声明 质量保证 Evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ChAT on 10 µg of NIH/3T3 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
For short term storage (3-4 weeks) maintain at +2-8°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µL
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