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品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, M WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3123 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Tie-1 Antibody, CT 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA and 0.05% sodium azide. 应用 应用 This Anti-Tie-1 Antibody, C-terminus is validated for use in ELISA, WB for the detection of Tie-1. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1-10 μg/mL using ECL. NIH/3T3 cells can be used as positive control. The predicted size of Tie-1 is ~135-140 kDa.
ELISA: (1:10,000 -1:100,000) using 50-100 ng of control peptide per well.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 20 amino acid peptide sequence within the cytoplasmic, C-terminus of human Tie-1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes human Tunica internal endothelial cell kinase (Tie-1), also described as tyrosine protein kinase receptor. Tie-1 (human 1138 aa; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.
SPECIES REACTIVITIES: The peptide sequence used is 100% conserved in mouse, rat, and bovine. Antibody crossreactivity in other species is not established.品种反应性 RatBovineHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005424.2
基因符号 - EC 2.7.10.1
- TIE1
- JTK14
- TIE
UniProt编号 - P35590
UniProt汇总 FUNCTION: SwissProt: P35590 # Probable protein tyrosine-kinase transmembrane receptor.
SIZE: 1138 amino acids; 125090 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Specifically expressed in developing vascular endothelial cells.
SIMILARITY: SwissProt: P35590 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. & Contains 3 EGF-like domains. & Contains 3 fibronectin type-III domains. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze and thaw. 包装信息 数量 50 µg
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