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merck millipore,默克密理博,05-1028,Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2
产品名称：Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2
产品型号：05-1028
Detect phospho-MAPKAPK2 (Thr334) using this Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2 validated for use in IF, ELISA & WB.
merck millipore,默克密理博,05-1028,Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ELISA M Purified Monoclonal Antibody 描述 产品目录编号 05-1028 商名 - Upstate
描述 Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2 Alternate Names - Mitogen-activated protein kinase-activated protein kinase 2
- MAPK-activated protein kinase 2
- MAPKAP kinase 2
背景信息 MAPKAP Kinases are Ser/Thr kinases activated by phosphorylation by the SAPK2/3, or p38 family of stress-activated kinases. MAPKAP Kinase 2 and 3 phosphorylate substrates with the sequence H-X-R-X-X-S where H is any hydrophobic residue, including CREB (Ser 133) and HSP27. MAPKAP kinases are similar to Rsk kinases, which are activated by mitogen-signaling pathways, rather than stress-signaling pathways. MAPKAP Kinase 2 is involved in many cellular processes that include inflammation, stress, and prolifertion. 产品信息 格式 Purified 控制 - HeLa nuclear extract
演示 Protein G purified mouse monoclonal in PBS with 0.05% NaN3. 应用 应用 Detect phospho-MAPKAPK2 (Thr334) using this Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2 validated for use in IF, ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Immunofluorescence Confocal Microscopy Analysis:
NIH/3T3 cells were fixed, permeabilized, and stained using Anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (Red). Cells were co-stained for actin and nuclear staining with Phalloidin, AlexaFluor® 488 conjugate (Green) and DAPI (Blue), respectively生物信息 免疫原品种 KLH-conjugated synthetic peptide containing the sequence and surrounding amino acids phosphorylated Thr334 of human, mouse, rat, bovine and chicken MAPKAP Kinase 2. Immunizing peptide shared 66% homology with phospho-MAPKAP-K3 at Thr313. 表位 Thr334 克隆 9F10.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Detects MAPKAP Kinase 2 only when phosphorylated on Thr334. 同种型 IgG1κ 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, Rat. Other species have not been tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_116584
Entrez基因汇总 This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. 基因符号 - MAPKAPK-2
修改 - Phosphorylation
纯化方法 Protein G purfied UniProt编号 - P49137
UniProt汇总 FUNCTION: Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue By similarity. Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
Catalytic activity ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation Seems to be activated by two distinct pathways: the first involves the stimulation of p42/p44 MAPK by growth factors, the second, triggered by stress and heat shock, depends on the activation of MPK2 and upstream MAPKK/MAPKKK.
SUBUNIT: structure Interacts with PHC2.
SPECIFITY: Expressed in all tissues examined.
PTM:Phosphorylated and activated by MAP kinase.
SIMILARITY: Belongs to the protein kinase superfamily. CAMK产品使用声明 质量保证 Routinely evaluated by Western Blot
Western Blot Analysis:
This lot detected MAPKAPK2 when phosphorylated on Thr334 at 1:1,000 dilution in PC12 lysates resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance包装信息 数量 100 µg
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