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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB122S 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dopamine Antibody 产品信息 格式 Serum 演示 Neat rabbit antisera with 0.1% sodium azide. 应用 应用 Detect Dopamine using this Anti-Dopamine Antibody validated for use in IH. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry at 1:500-1:1,000.
PERFUSION MIXTURE AND PROTOCOL FOR IMMUNOCYTOCHEMICAL DETECTION OF DOPAMINE: See suggested protocols.
AB122S should be used at dilutions of 1:500-1:1,000 using Tris-MBS containing 0.5% Trition and 1% normal goat serum. The antibody should be incubated 15 hours at +4°C or room temperature. Final working dilutions must be determined by end user.
Post Treatment:
The immunocytochemistry procedure is then continued with the PAP method or the avidin-biotin peroxidase system. Note: since MBS is known to inhibit PAP or avidin-biotin peroxidase, it should be omitted for this step. 40 mM Tris-HCl with 9% NaCl adjusted to pH 7.6 (using concentrated HCl) should be used.
Notations:
MBS is essential in the pre-wash and the glutaraldehyde solution since this antioxidant protects the dopamine molecule from being released.
*Protocol modified from: McRae-Degueurce, A., et al. (1986). Brain Research 376:217-219.生物信息 免疫原品种 Dopamine-BSA-Glutaraldehyde conjugate 宿主 Rabbit 特异性 Dopaminergic neurons of substantia nigra and of the A10 region as well as dopaminergic nerve terminals of locus coeruleus. Immunostaining is abolished by pre-incubation with 10-100 μg dopamine-BSA-Glutaraldehyde conjugate per mL of diluted antibody. Repeated absorptions of the serum with nonadrenaline coupled to BSA does not alter the immunocytochemical staining pattern. 品种反应性 RatHuman 抗体类型 Polyclonal Antibody 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
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下一件merck millipore产品：merck millipore,默克密理博,05-182,Anti-FAK Antibody, clone 2A7
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