- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
格式 检测方法 Liquid, Antibody Diluent Chemiluminescence, colorimetric, or fluorescence 描述 概述 SignalBoost™ Immunoreaction Enhancer Kit is designed to enhance the desired signal in assays such as immunoblotting, dot blotting, and ELISA. This kit significantly improves sensitivity and discrimination when assessing protein targets of low immunoreactivity or low expression levels. Enhancement of signal is especially marked for low affinity antibodies. It replaces conventional antibody diluents with Solution 1, which is used to dilute primary antibodies and Solution 2, which is used to dilute secondary antibodies. No additional steps, materials, or increase of assay time is required.
• Increases signal: minimizes background resulting in high signal to noise ratios
• Versatile: can be used with nitrocellulose or PVDF membranes and is compatible with chemiluminescent or colorimetric detection systems
• Convenient: ready-to-use format, no dilution necessary产品目录编号 407207 品牌系列 Calbiochem®
应用数据 
Detection of protein kinase C by immunoblotting. Samples: whole cell lysate from K562 cells. Immunoblotting conditions: nitrocellulose membrane blocked overnight with 5% milk in PBST; all washes were performed with PBST. Primary antibody: Anti-PKC (Ab-2) Mouse mAb (MC5) (Cat. No. OP74) diluted 1:1000 in 5% milk/PBST (lane A) or in Solution 1 (lane B) (incubation for 1 h ). Secondary antibody: Anti-Mouse IgG diluted 1:10,000 in 5% milk/PBST (lane A) or Solution 2 (lane B). Detection: chemiluminescence (30 s exposure).
ELISA results generated with and without dilution of antigen and detection antibody using SignalBoost™ reagent.产品信息 检测方法 Chemiluminescence, colorimetric, or fluorescence 形式 20 Miniblots or 1600 cm² 格式 Liquid, Antibody Diluent 试剂盒包含 Solution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol. 产品使用声明 预定用途 SignalBoost™ Immunoreaction Enhancer Solution is a reactive reagent for use in immunoassays, and was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as immunoblotting and ELISA (enzyme-linked immunosorbent assay). It can be used in a range of immunoassays. 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 +2°C to +8°C
存储条件 Upon receipt, store the entire contents of the kit at 4°C. Protect from light. Following initial use, this product is stable for up to 1 year at 4°C. 避光 Protect from light
无需冷冻 No
补充信息 试剂盒包含 Solution 1 for Primary Antibodies, Solution 2 for Secondary Antibodies, and a user protocol.
上一件merck millipore产品：merck millipore,默克密理博,5920-OP,OmniPur Lithium Chloride, 8 M - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,MSBVN1210,MultiScreenHTS BV，1.2 µm，无色，非无菌
相关产品
merck millipore,默克密理博,AB122S,Anti-Dopamine Antibody
merck millipore,默克密理博,05-182,Anti-FAK Antibody, clone 2A7
merck millipore,默克密理博,ST1549,Anti-FASN Mouse mAb (3F2-1F3)
merck millipore,默克密理博,5920-OP,OmniPur Lithium Chloride, 8 M - Calbiochem
merck millipore,默克密理博,MSBVN1210,MultiScreenHTS BV，1.2 µm，无色，非无菌
merck millipore,默克密理博,MAB3786,Anti-APC Antibody, CT, clone C-APC 28.9
merck millipore,默克密理博,07-529,Anti-KIF21A Antibody
merck millipore,默克密理博,MABC188,Anti-Deoxycytidine Kinase Antibody, clone 9D4
