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merck millipore,默克密理博,07-958,Anti-SUV39H1 Antibody
产品名称：Anti-SUV39H1 Antibody
产品型号：07-958
Anti-SUV39H1 Antibody is a Rabbit Polyclonal Antibody for detection of SUV39H1 also known as H3-K9-HMTase 1, Histone H3-K9 methyltransferase 1, Lysine N-methyltransferase 1A & has been validated in WB More>>
merck millipore,默克密理博,07-958,Anti-SUV39H1 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-958 Replaces MABE552 描述 Anti-SUV39H1 Antibody Alternate Names - suppressor of variegation 3-9 homolog 1 (Drosophila)
- H3-K9-HMTase 1
- Histone H3-K9 methyltransferase 1
- Lysine N-methyltransferase 1A
- Position-effect variegation 3-9 homolog
- Su(var)3-9 homolog 1
- Suppressor of variegation 3-9 homolog 1
- histone-lysine N-methyltransferase, H3 lysine-9 specific 1
- suppressor of variegation 3-9 (Drosophila) homolog 1
- suppressor of variegation 3-9 (Drosophila) homolog 1
背景信息 Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2. Suv39H1 is a nuclear protein that acts as a H3K9 specific methyltransferase (HMT). In addition to its role in gene silencing, Suv39H1 is also thought to be involved in heterochromatin organization, chromosome segregation, and mitotic progression, and the epigenetic regulation of telomere length in mammalian cells. 产品信息 格式 Affinity Purified 控制 - U2OS lysate
演示 Affinity purified rabbit polyclonal in 0.1 M Tris-Glycine (pH 7.4), 15 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-SUV39H1 Antibody is a Rabbit Polyclonal Antibody for detection of SUV39H1 also known as H3-K9-HMTase 1, Histone H3-K9 methyltransferase 1, Lysine N-methyltransferase 1A & has been validated in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
A previous lot of this antibody has been used to immunoprecipitate SUV39H1 from U2OS cells.生物信息 免疫原品种 GST-tagged fusion protein including amino acids 82-415 of human SUV39H1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes SUV39H1. 品种反应性 Human Species Reactivity Note Human. Does not react with mouse, rat, or bovine SUV39H1, despite significant sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003164
Entrez基因汇总 This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. [provided by RefSeq].
基因符号 - SUV39H
- EC 2.1.1.43
- KMT1A
- MG44
- OTTHUMP00000024298
非反应性品种 - Rat
- Bovine
- Mouse
纯化方法 Antigen affinity purified UniProt编号 - O43463
UniProt汇总 FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Ref.15 Ref.19 Ref.22 Ref.23
Catalytic activity S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.7
Enzyme regulation Inhibited by S-adenosyl-L-homocysteine. Ref.20
SUBUNIT STRUCTURE: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR. Ref.22 Ref.1 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.16 Ref.17 Ref.18 Ref.24 Ref.25
SUBCELLULAR LOCATION: Nucleus. Centromere. Note= Associates with centromeric constitutive heterochromatin. Ref.6
DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
DOMAIN: Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. Ref.17
PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function. Ref.6 Ref.21 Ref.26 Ref.27
SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 chromo domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
产品使用声明 质量保证 Western Blot Analysis: 0.1 μg/ml dilution of this lot detected SUV39H1 on 10 μg of U2OS lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. 包装信息 数量 100 µg
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