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品种反应性 主要应用 宿主 格式 抗体类型 Ca, H, M, Chp, Rhesus Macaque WB, ICC, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB10181 描述 Anti-Pumilio-2 (PUM2) Antibody Alternate Names - pumilio (Drosphila) homolog 2
- pumilio homolog 2 (Drosophila)
- pumilio homolog 2
背景信息 PUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts. 产品信息 格式 Affinity Purified 控制 - Mouse embryonic stem cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Detect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Pumilio-2 near the N-terminus. 品种反应性 CanineHumanMouseChimpanzeeRhesus Macaque Species Reactivity Note Demonstrated to react with human and mouse.
Predicted to react with chimpanzee, rhesus monkey, and canine based on 100% sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NP_056132
基因符号 - PUMH2
- PUM2
- PUML2
纯化方法 Affinity Purfied UniProt编号 - Q8TB72
UniProt汇总 FUNCTION: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
SUBUNIT STRUCTURE: Homodimer; homodimerizes in vitro. Interacts with DAZ, DAZL and NANOS1 via its pumilio repeats. Binds to a RNA consensus sequence, that is related to the Nanos Response Element (NRE), a 16 bp sequence found in the 3'-UTR of the Drosophila hb mRNA. Also binds to the NRE.
SUBCELLULAR LOCATION: Cytoplasm Probable.
TISSUE SPECIFICITY: Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
INDUCTION: Down-regulated in keratinocytes upon UV B radiation.
DOMAIN: The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half donut. The number as well as the specific sequence of the repeats determine the specificity for target mRNAs By similarity.
SEQUENCE SIMILARITIES: Contains 1 PUM-HD domain.
Contains 8 pumilio repeats.产品使用声明 质量保证 Evaluated by Western Blot on mouse embryonic stem cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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