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merck millipore,默克密理博,71345,Origami™ 2(DE3) Competent Cells - Novagen
产品名称：Origami™ 2(DE3) Competent Cells - Novagen
产品型号：71345
Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.
merck millipore,默克密理博,71345,Origami™ 2(DE3) Competent Cells - Novagen
- 产品介绍
描述 概述 Origami™ 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB (StrR, TetR) 产品目录编号 71345 品牌系列 Novagen®
产品信息 部件 保证高效 2 × 10⁶ cfu/µg 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 ≤ -70°C
无需冷冻 No
布鲁克黑文许可证 This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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