merck millipore,默克密理博,05-689,Anti-HP1α Antibody, clone15.19s2

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,05-689,Anti-HP1α Antibody, clone15.19s2
产品名称：Anti-HP1α Antibody, clone15.19s2
产品型号：05-689
Anti-HP1α Antibody, clone15.19s2 is a Mouse Monoclonal Antibody for detection of HP1α also known as HP1Hs alpha, Heterochromatin protein 1 homolog alpha & has been validated in ChIP, ICC, IHC, IP & WB More>>

merck millipore,默克密理博,05-689,Anti-HP1α Antibody, clone15.19s2

产品介绍

merck millipore,默克密理博,05-689,Anti-HP1α Antibody, clone15.19s2

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt, H, M	ChIP, ICC, IHC, WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-689
Replaces	MABE479
品牌系列	Upstate
商名	Upstate
描述	Anti-HP1α Antibody, clone15.19s2
Alternate Names	heterochromatin protein 1-alpha Antigen p25 HP1 alpha HP1Hs alpha Heterochromatin protein 1 homolog alpha chromobox homolog 5 (Drosophila HP1 alpha) chromobox homolog 5 (HP1 alpha homolog, Drosophila) Drosophila)
背景信息	Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation.

产品信息
格式	Purified
控制	HeLa, NIH/3T3 and COS cell lysates.
演示	Purified mouse monoclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%.

应用
应用	Anti-HP1α Antibody, clone15.19s2 is a Mouse Monoclonal Antibody for detection of HP1α also known as HP1Hs alpha, Heterochromatin protein 1 homolog alpha & has been validated in ChIP, ICC, IHC, IP & WB.
主要应用	Chromatin Immunoprecipitation (ChIP) Immunocytochemistry Immunohistochemistry Western Blotting
应用说明	Immunohistochemistry: A previous lot of this antibody has been reported by an independent laboratory to detect HP1a in NIH/3T3 nuclei (Ayyanathan, K., 2003). Immunoprecipitation/Chromatin Immunoprecipitation: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate HP1α from nuclear extracts and formalin-cross-linked chromatin (Ayyanathan, K., 2003).

生物信息
免疫原品种	GST fusion protein corresponding to residues 2-191 of human HP1α protein.
克隆	15.19s2
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes HP1α
同种型	IgG1
品种反应性	VertebratesHumanMouse
Species Reactivity Note	Human, mouse and vertebrates
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_012117
Entrez基因汇总	Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.[supplied by OMIM]
基因符号	HP1 CBX5 HP1A HP1Hs-alpha HP1-ALPHA
纯化方法	Protein G chromatography
UniProt编号	P45973
UniProt汇总	FUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. SIZE: 191 amino acids; 22225 Da SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127. SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis. SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains.

产品使用声明
质量保证	Routinely evaluated by western blot in acid-extracted HeLa cell lysates. Western Blot Analysis: 0.5-2 µg/mL of this lot detected HP1α protein in acid-extracted HeLa cell lysates.
销售限制	Due to license agreement restrictions, this product cannot be purchased for resale.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.