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品种反应性 主要应用 宿主 格式 抗体类型 R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5669 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Spinophilin Antibody Alternate Names - Neurabin-II
产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in 0.02M phosphate buffer containing 0.25M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 Anti-Spinophilin Antibody detects level of Spinophilin & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western blot: 0.05 - 0.1 μg/mL using ECL on rat brain lysate. Reacts with a band of ~140 kDa.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from rat Spinophilin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Spinophilin 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_032595.3
Entrez基因汇总 Spinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1; see MIM 176875) and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system (Allen et al., 1997 [PubMed 9275233]).[supplied by OMIM] 基因符号 - SPINO
- PPP1R9B
- Spinophilin
- PPP1R9
- Neurabin-2
- PPP1R6
- FLJ30345
- spinophilin
- Neurabin-II
UniProt编号 - Q96SB3
UniProt汇总 FUNCTION: SwissProt: Q96SB3 # Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha- adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity).
SIZE: 815 amino acids; 89192 Da
SUBUNIT: Possibly exists as an homodimer, homotrimer or an homotetramer. Interacts with F-actin, PPP1CA, neurabin-1, TGN38 and D(2) dopamine receptor. Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A, PPP1R2, RASGFR1 and TIAM1.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Cell projection, dendritic spine (By similarity). Cell junction, synapse. Note=Enriched at synapse and cadherin- based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity).
PTM: Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1 (By similarity).
SIMILARITY: SwissProt: Q96SB3 ## Contains 1 PDZ (DHR) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted for up to 6 months after date of receipt. 包装信息 数量 100 µg
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