- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
描述 概述 The pET-41a-c(+) and pET-42a-c(+) vectors incorporate the schistosomal glutathione-S-transferase (GST; GST•Tag) coding sequence as a fusion partner. The GST•Tag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners (Smith 1998). When expressed in a soluble, properly folded form, GST•Tag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. The pET-41 and -42 series feature the powerful T7lac promoter, and encode the GST•Tag (220 aa) sequence, proteolytic sites, His•Tag (6 aa) sequence, and S•Tag (15 aa) sequence.
In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow removal of 100% of the vector-encoded sequences and the generation of native proteins with their authentic N-terminal residues. Unfused proteins can be produced by using the Nde I cloning site. A version of pET-41 is available as a linearized vector prepared for ligation-independent cloning (LIC) of PCR products.
Another pET vector with the GST•Tag sequence is pET-49b(+). Please see the website description for more information. The His•Tag and S•Tag sequences enable alternative protein detection and purification procedures to be performed. For example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.
The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB239). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase.
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.
Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.产品目录编号 70561 品牌系列 Novagen®
参考 参考 Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31.
储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Standard Handling
储存 ≤ -70°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
上一件merck millipore产品：merck millipore,默克密理博,ABF131,Anti-phospho TIE2/TEK Antibody (Tyr992)
下一件merck millipore产品：merck millipore,默克密理博,MAB80144,Anti-Newcastle Disease Virus Antibody
相关产品
merck millipore,默克密理博,MAMCS9610,MultiScreen 96 孔培养板，无色，无菌
merck millipore,默克密理博,480748,Neuronal Differentiation Inducer, Isobavachin - Calbiochem
merck millipore,默克密理博,MAB2130,Anti-ICAM-1 Antibody, clone W-CAM-1
merck millipore,默克密理博,AB3582P,Anti-Cyclic Nucleotide-Gated Channel 1 Antibody, CT
merck millipore,默克密理博,ABF131,Anti-phospho TIE2/TEK Antibody (Tyr992)
merck millipore,默克密理博,MAB80144,Anti-Newcastle Disease Virus Antibody
merck millipore,默克密理博,FCMAB261P,Milli-Mark™ Anti-Eck/EphA2-PE Antibody, clone D7
merck millipore,默克密理博,MAB2251,Anti-Integrin β1 Antibody, clone B3B11
merck millipore,默克密理博,513050,PDE10A Inhibitor, Cpd15 - Calbiochem
merck millipore,默克密理博,AP160P,Rabbit Anti-Mouse IgG Antibody, HRP conjugate