merck millipore,默克密理博,69999,T7•Tag Antibody AP Conjugate

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merck millipore,默克密理博,69999,T7•Tag Antibody AP Conjugate
产品名称：T7•Tag Antibody AP Conjugate
产品型号：69999

merck millipore,默克密理博,69999,T7•Tag Antibody AP Conjugate

产品介绍

merck millipore,默克密理博,69999,T7•Tag Antibody AP Conjugate

描述

概述

The T7•Tag AP Conjugate is useful as a single detection reagent and eliminates the need for second antibodies or streptavidin conjugates. The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

The T7•Tag^® Antibody is a mouse monoclonal antibody (IgG_2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

This antibody is also available labeled with biotin and as a direct conjugate with horseradish peroxidase.

Specificity	T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/Isotype	Mouse monoclonal IgG_2b
Cross-reactivity	Negligible with bacterial, insect or mammalian cell lysates
Sensitivity	1–10 ng (Western blot developed with chromogenic substrates) < 1 ng (developed with chemiluminescent substrates)
Form	Stabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution	1:5,000–1:10,000