merck millipore,默克密理博,07-1477,Anti-Rsk1 Antibody

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merck millipore,默克密理博,07-1477,Anti-Rsk1 Antibody
产品名称：Anti-Rsk1 Antibody
产品型号：07-1477
Anti-Rsk1 Antibody is an antibody against Rsk1 for use in WB, IP, ELISA.

merck millipore,默克密理博,07-1477,Anti-Rsk1 Antibody

产品介绍

merck millipore,默克密理博,07-1477,Anti-Rsk1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ELISA, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-1477
Replaces	04-417
描述	Anti-Rsk1 Antibody
Alternate Names	Ribosmal protein S6 kinase, 90kDa, polypeptide 1 90 kDa ribosomal protein S6 kinase 1 MAP kinase-activated protein kinase 1 Ribosomal S6 kinase 1 Ribosomal protein S6 kinase alpha-1 S6K-alpha 1 p90-RSK 1 Ribosomal protein S6 kinase alpha 1 Ribosomal protein S6 kinase, 90kD, 1 90kD 1 Ribosmal protein S6 kinase 90kDa
背景信息	Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.

产品信息
格式	Affinity Purified
控制	NIH/3T3 cell lysate
演示	Purified rabbit polyclonal IgG in buffer containing TBS with 0.01% Sodium Azide in 50% glycerol.

应用
应用	Anti-Rsk1 Antibody is an antibody against Rsk1 for use in WB, IP, ELISA.
主要应用	ELISA Western Blotting
应用说明	Immunoprecipitation: 4 µg of a previous lot of this antibody was used per 1500 µg of crude protein sample in Immunoprecipitation. ELISA: Recommended. Optimal working dilutions must be determined by end user. Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.

生物信息
免疫原品种	Synthetic peptide corresponding to the c-terminal sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes p90 RSK1.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse and rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_001006665.1
Entrez基因汇总	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
基因符号	EC 2.7.11.1 RPS6KA1 HU-1 MAPKAPK1A RSK pp90RSK1 RSK-1 p90S6K RSK1
纯化方法	Affinity Purfied
UniProt编号	Q15418
UniProt汇总	FUNCTION: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Activated by multiple phosphorylations on threonine and serine residues. SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation. PTM: Autophosphorylated on Ser-380, as part of the activation process. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. SIMILARITY: Contains 1 AGC-kinase C-terminal domain. SIMILARITY: Contains 2 protein kinase domains.

产品使用声明
质量保证	Routinely evaluated by Western Blot on NIH/3T3 lysates. Western Blot Analysis: 1:500-1:1,000 dilution of this lot detected RSK1 on 10 μg of NIH/3T3 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.