merck millipore,默克密理愽
  • merck millipore,默克密理博,69820,pT7Blue T-Vector - Novagen

  • merck millipore,默克密理博,69820,pT7Blue T-Vector - Novagen

    产品名称：pT7Blue T-Vector - Novagen
    产品型号：69820


    merck millipore,默克密理博,69820,pT7Blue T-Vector - Novagen

  • 产品介绍
  • merck millipore,默克密理博,69820,pT7Blue T-Vector - Novagen

    描述
    概述 The pT7Blue T-Vector is designed for simplified cloning of PCR products. Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3' A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue vector has been specifically constructed for this application and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3' dT residues at each end. The T-Vector is provided ready to ligate with DNA amplified with any DNA polymerase that leaves single 3' dA overhangs. Target inserts having other types of ends, such as reaction products of proofreading polymerase or restriction fragments, can be easily prepared for T-cloning with the Single dA™ Tailing Kit (Cat. No. 69282-3). pT7Blue contains the pUC19 backbone (including high-copy number origin or replication and lac sequences), a T7 promoter, f1 origin of replication, and a modified multiple cloning region.
    产品目录编号 69820
    品牌系列 Novagen®
    产品信息
    部件
    声明 This product is only available in Japan.
    储存和货运信息
    货运代码 Shipped with Blue Ice or with Dry Ice
    毒性 Standard Handling
    储存 -20°C
    无需冷冻 No

    merck millipore,默克密理博,69820,pT7Blue T-Vector - Novagen

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