merck millipore,默克密理博,05-952,Anti-RNA Polymerase II Antibody, CTD, clone 8WG16

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merck millipore,默克密理博,05-952,Anti-RNA Polymerase II Antibody, CTD, clone 8WG16
产品名称：Anti-RNA Polymerase II Antibody, CTD, clone 8WG16
产品型号：05-952
Use Anti-RNA Polymerase II Antibody, CTD, clone 8WG16 (Mouse Monoclonal Antibody) validated in ChIP, ICC, IP, WB to detect RNA Polymerase II also known as DNA directed RNA polymerase II polypeptide A.

merck millipore,默克密理博,05-952,Anti-RNA Polymerase II Antibody, CTD, clone 8WG16

产品介绍

merck millipore,默克密理博,05-952,Anti-RNA Polymerase II Antibody, CTD, clone 8WG16

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Yeast (S. cerevisiae), H	WB, ICC	M	Ascites	Monoclonal Antibody

描述
产品目录编号	05-952
品牌系列	Upstate
商名	Upstate
描述	Anti-RNA Polymerase II Antibody, CTD, clone 8WG16
Alternate Names	polymerase (RNA) II (DNA directed) polypeptide A, 220kDa DNA directed RNA polymerase II polypeptide A DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220<br />kd subunit DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit RNA polymerase II subunit B1 polymerase (RNA) II (DNA directed) polypeptide A (220kD) polymerase (RNA) II (DNA directed) polypeptide A (220kD) polymerase (RNA) II (DNA directed) polypeptide A
背景信息	As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity

产品信息
格式	Ascites
控制	HeLa nuclear extracts. Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
演示	Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.

应用
应用	Use Anti-RNA Polymerase II Antibody, CTD, clone 8WG16 (Mouse Monoclonal Antibody) validated in ChIP, ICC, IP, WB to detect RNA Polymerase II also known as DNA directed RNA polymerase II polypeptide A.
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation: Recommended by an independent laboratory. Immunocytochemistry: Recommended by an independent laboratory.

生物信息
免疫原品种	RNA Polymerase II from wheat germ extract. Clone 8WG16.
克隆	8WG16
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Recognizes RNA Polymerase II, Mr 220 kDa.
品种反应性	Yeast (S. cerevisiae)Human
Species Reactivity Note	Human and yeast. Broad species cross-reactivity is expected based on sequence conservation of immunogen.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_000928
Entrez基因汇总	This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
基因符号	hRPB220 POLR2A POLRA RpIILS POLR2 RPB1 MGC75453 hsRPB1 RPOL2 RPBh1 RPO2
纯化方法	Unpurified
UniProt编号	P24928
UniProt汇总	FUNCTION: SwissProt: P24928 # DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.

产品使用声明
质量保证	Routinely evaluated by western blot. Western Blot Analysis: 1:500-1:2000 dilution of this lot detected RNA Polymerase II in HeLa nuclear extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.