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品种反应性 主要应用 宿主 格式 抗体类型 Vrt, B, H, M, Po, R WB M Purified Monoclonal Antibody 描述 产品目录编号 05-173 Replaces 04-1158 品牌系列 Upstate
商名 - Upstate
描述 Anti-Calmodulin Antibody Alternate Names - phosphorylase kinase, delta subunit
- calmodulin 1
- calmodulin 1 (phosphorylase kinase, delta)
- delta)
- phosphorylase kinase
背景信息 Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.产品信息 格式 Purified 控制 - Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Use 20 μg per lane for minigels.
演示 Purified mouse IgG1 in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution. 应用 应用 Detect Calmodulin using this Anti-Calmodulin Antibody validated for use in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
4 μg of a previous lot immunoprecipitated calmodulin from A431 RIPA cell lysate. Radioimmunoassay:
Use 10 μg/mL to detect calmodulin in the range of 200-300 ng.生物信息 免疫原品种 Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin. 表位 a.a. 128-148 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Specific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin. 同种型 IgG1 品种反应性 VertebratesBovineHumanMousePigRat Species Reactivity Note Also reacts to wheat germ. Wide cross-reactivity expected. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_008819
Entrez基因汇总 Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.[supplied by OMIM] 基因符号 - CAM1
- CALM1
- CALML2
- CALM3
- CALM
- CaM
- CALM2
- CAM2
- CAMC
- CAM3
- DD132
- CAMIII
- PHKD
- CAMI
- CAMB
- CAM
纯化方法 Protein G chromatography UniProt编号 - P62158
UniProt汇总 FUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
SUBUNIT STRUCTURE: Interacts with MYO1C By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY.
SUBCELLULAR LOCATION: Spindle. Note= Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. Ref.20
PTM Ubiquitination results in a strongly decreased activity By similarity.
Phosphorylation results in a decreased activity By similarity.
MISCELLANEOUS This protein has four functional calcium-binding sites.
SEQUENCE SIMILARITIES Belongs to the calmodulin family.
Contains 4 EF-hand domains.
Sequence caution The sequence CAA36839.1 differs from that shown. Reason: Erroneous gene model prediction.产品使用声明 质量保证 Evaluated on human A431 carcinoma cells, RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.
Western Blot Analysis: 0.1-1 μg/mL of this antibody detected calmodulin in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,ABC287,Anti-FANCA Antibody
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