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描述 概述 The Perfect Protein™ HRP Western Blot Kit includes an HRP Conjugate in addition to the Perfect Protein™ Western Markers (Cat. No. 69959). For a version containing prestained proteins for tracking electrophoresis and Western transfer, please refer to our complete listing of Trail Mix™ Western Markers and Blot Kits.
A.Two parallel blots were incubated with primary antibodies for an expressed protein, then incubated with Anti-Mouse IgG AP Conjugate and processed using chemiluminescent detection. The S-protein AP Conjugate was included with secondary antibody for the blot on the left. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 10 ng induced cell extract; 3, 1 µg uninduced cell extract.
B.Blot contains the Perfect Protein Western Markers plus purified GUS expressed in insect cells incubated with a GUS-specific monoclonal antibody, then incubated with Anti-Mouse IgG HRP Conjugate and S-protein HRP Conjugate and detected with chemiluminescent HRP substrate. Lanes: 1, 5 µl Perfect Protein Western Markers; 2, 800 ng purified GUS protein.产品目录编号 69078 品牌系列 Novagen®
特点及优点 • Detect markers simply by adding the S-protein (AP or HRP Conjugate) or His•Tag® Monoclonal Antibody to the same incubation used for sample detection.
• S-protein conjugate (included in kits) does not interfere with antibodies or streptavidin detection.
• Can be used with colorimetric and chemiluminescent AP or HRP substrates.
• Recombinant, unmodified markers give sharp, accurately sized bands.
• Markers are supplied at the working dilution in gel loading buffer; concentration optimized for Western detection.
• Protein sizes are 15, 25, 35, 50, 75, 100, and 150 kDa.
产品信息 部件 形式 Liquid 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
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下一件merck millipore产品：merck millipore,默克密理博,03-34-0051,Calpeptin - CAS 117591-20-5 - Calbiochem
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