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merck millipore,默克密理博,16-103,Anti-Phosphotyrosine Antibody, clone 4G10, Biotin Conjugate
产品名称：Anti-Phosphotyrosine Antibody, clone 4G10, Biotin Conjugate
产品型号：16-103
Anti-Phosphotyrosine Antibody, clone 4G10, Biotin Conjugate detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is published in peer reviewed journals and valida More>>
merck millipore,默克密理博,16-103,Anti-Phosphotyrosine Antibody, clone 4G10, Biotin Conjugate
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A WB, ICC M Biotin Monoclonal Antibody 描述 产品目录编号 16-103 品牌系列 Upstate
商名 - Upstate
描述 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate 概述 Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat.# 05-321), cross-linked to biotin. 背景信息 Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
Anti-phosphotyrosine monoclonal antibody, clone 4G10® (cat. # 05-321), cross-linked to biotin.产品信息 格式 Biotin 控制 - Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
演示 PBS containing 0.05% sodium azide. 应用 应用 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is published in peer reviewed journals and validated for use in IC, IP, WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation:
4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells.
Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry:
5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].
Application Notes
For use in applications in which a biotin conjugate is advantageous, such as WB and IC.生物信息 免疫原品种 Phosphotyramine-KLH. 表位 N/A 克隆 4G10® 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes tyrosine-phosphorylated proteins from all species. 同种型 IgG2bκ 品种反应性 All Species Reactivity Note Human. Expected to cross-react with rat and mouse. 抗体类型 Monoclonal Antibody 修改 - Phosphorylation
纯化方法 Protein G purfied 产品使用声明 质量保证 Routinely evaluated by Western Blot on EGF treated A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 9 months at 2-8°C from date of shipment. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 包装信息 数量 100 µg
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