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描述 概述 Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. Referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune systems response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation. 产品目录编号 654245 品牌系列 Calbiochem®
同义词 rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α 参考 参考 Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.产品信息 形式 Lyophilized 配方 Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α. Molar Mass 17000 生物信息 生物活性 ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666) 纯度 ≥97% by SDS-PAGE 理化信息 污染物 Endotoxin: ≤100 pg/µg TNF-α 溶解度 Sterile PBS containing ≥0.1% HSA or BSA 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 ≤ -70°C
无需冷冻 No
特殊指南 Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
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