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merck millipore,默克密理博,553009,PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb
产品名称：PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb
产品型号：553009
This PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).
merck millipore,默克密理博,553009,PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes c-Raf phosphorylated at Tyr340 and Tyr341 in HEK293 cells overexpressing c-Raf. Note: 1 T = 1 test. Sufficient for 10 Western miniblots. 产品目录编号 553009 品牌系列 Calbiochem®
应用数据 
Detection of human c-Raf, phosphorylated on Tyr340, by immunoblotting. Samples: Whole cell lysate from HEK293 cells overexpressing c-Raf left unstimulated (lane 1) or stimulated with active Src (lane 2-5). Primary antibody: PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb (Cat. No. 553009) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.参考 参考 Yan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
Mason, C.S. et al. 1999. EMBO J. 18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
Moelling, K. et al. 1984. Nature 312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.产品信息 形式 Liquid 配方 In phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1). 正控制 HEK293 cells over expressing c-Raf, phosphorylated in vitro by Src. 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1) 免疫原品种 Human 宿主 Rabbit 同种型 IgG 品种 human, mouse, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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