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描述 概述 The ProteoExtract® Subcellular Proteome Extraction Kit, Mini is a tool for the differential extraction of proteins from mammalian cells according to their subcellular localization. The sequential extraction steps yield proteins in their native state, making it highly suited for demanding proteomics applications. Sample size: 3-5x106 or 25-50 mg tissue. 产品目录编号 539791 品牌系列 Calbiochem®
同义词 S-PEK Kit, Mini 参考 参考 Yuan, X., et al. 2002. Electrophoresis 23, 1185.
Butcher, B.A., et al. 2001. J. Immunol. 167, 2193;
Ott, V., et al. 2001. Pharmacogenomics J. 1, 142.
Allen, L., et al. 2000. Nature 405, 819.
Rabilloud, T. 2000. Anal. Chem. 72, 48A.
Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93.
Reymond, et al. 1997. Electrophoresis 18, 2842.
Laemmli, U. K. 1970. Nature 227, 680.
Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265.产品信息 形式 10 Extractions 试剂盒包含 Wash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol. 生物信息 样品类型 Mammalian tissues and cultured cells 产品使用声明 预定用途 The Calbiochem® ProteoExtract® Subcellular Proteome Extraction Kit Mini (S-PEK) is intended for the differential extraction of proteins from mammalian cells according to their subcellular localization. The procedure yields proteins in their native state making it highly suited for demanding proteomics applications including enzyme activity assays (e.g. reporter gene assays) and subcellular redistribution assays to monitor protein shuttling (e.g. signaling proteins). 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 +2°C to +8°C
存储条件 Upon arrival store the components of the kit under the following conditions:
• Wash Buffer: Store at 4°C.
• Extraction Buffers I-III: Store at 4°C for up to 6 months. For long-term storage, freeze the buffers in convenient aliquots at -20°C.
• Extraction Buffer IV: Store at room temperature.
• Protease Inhibitor Cocktail: The cocktail is supplied in DMSO and can be stored at 4°C for up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C.
• Benzonase® Nuclease: Store at 4°C.无需冷冻 No
补充信息 试剂盒包含 Wash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
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