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merck millipore,默克密理博,442706,PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4)
产品名称:PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4)
产品型号:442706
This PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 Mouse mAb is validated for use in ELISA, WB, IP for the detection of MAP Kinase ERK1/ERK2 .
merck millipore,默克密理博,442706,PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4)
- 产品介绍
- merck millipore,默克密理博,442706,PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4)
重要规格表
宿主 M 描述 概述 Recognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204 in vanadate-treated A431 cells. Supplied with a control lysate. 产品目录编号 442706 品牌系列 Calbiochem®
同义词 Anti-MAPK1/2, Activated 产品信息 形式 Lyophilized 配方 100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells. 正控制 vanadate-treated A431 cells (supplied) 防腐剂 ≤0.1% sodium azide (antibody only) 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2 免疫原品种 Human 克隆 12D4 宿主 Mouse 同种型 IgG₁ 品种 human, mouse, rat 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Reconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
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