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检测方法 Colorimetric 描述 概述 A spectrophotometric assay kit that is specific for reduced glutathione (GSH) or total mercaptans. Absorbance is read at ~400 nm for GSH and ~356 nm for total mercaptens. Note: 1 T = 1 test. 产品目录编号 354102 品牌系列 Calbiochem®
参考 参考 Ji, L.L., and Fu, R. 1992. J. Appl. Physiol. 72, 549.
Anderson M.E. 1989. Enzymatic and chemical methods for the determination of
Glutathione; In: Glutathione: chemical, biochemical and medical aspects, Vol. A, Dolphin D., Poulson R. and Avramovic O. Eds., John Wiley and Sons, pp. 339-365.
Dolphin D., et al. 1989. Glutathione: Chemical, Biochemical and Medical Aspects, Vols A & B, J. Wiley and Sons.产品信息 检测方法 Colorimetric 形式 100 Tests 格式 Cuvette 试剂盒包含 A Chromogen, 30% NaOH, Buffer, and a user protocol. 生物信息 化验时间 1.5 h 样品类型 Cell lysates, tissue homogenates 理化信息 灵敏度 5.0 µM RSH 安全信息 R相 R: 34
Causes burns.S相 S: 23-26-27-36/37/39
Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Take off immediately all contaminated clothing.
Wear suitable protective clothing, gloves and eye/face protection.产品使用声明 预定用途 The Calbiochem® Glutathione Assay Kit makes it possible to assay for glutathione with only one sampling and one colorimetric measurement. A modification of this method makes it possible to assay other mercaptans. This alternate protocol is based on the measurement of substitution products that absorb maximally at 356 nm in the absence of reagent R2. Due to its ease of use, this method is well adapted for the quantification of glutathione and total mercaptans in a large number of biological samples. The main advantage of the method is that it does not require any enzyme as a reagent. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 +2°C to +8°C
存储条件 Upon arrival store the entire kit contents at 4°C.
• All solutions of reagents and buffer have been tightly sealed and are stable if properly stored between 4°C. Do Not Freeze. Protect from light.
• After removing the required amount of each reagent for immediate use, all bottles should be tightly closed and stored between 2-4°C. Do not leave the reagent bottles open or at room temperature.避光 Protect from light
无需冷冻 Yes
补充信息 试剂盒包含 A Chromogen, 30% NaOH, Buffer, and a user protocol.
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