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merck millipore,默克密理博,04-1464,Anti-Y14 Antibody, clone 1F12
产品名称：Anti-Y14 Antibody, clone 1F12
产品型号：04-1464
Use Anti-Y14 Antibody, clone 1F12 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect Y14 also known as Binder of OVCA1-1, RNA binding motif protein 8A, Ribonucleoprotein RBM8A.
merck millipore,默克密理博,04-1464,Anti-Y14 Antibody, clone 1F12
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 04-1464 描述 Anti-Y14 Antibody, clone 1F12 Alternate Names - ribonucleoprotein RBM8
- Binder of OVCA1-1
- RNA binding motif protein 8A
- RNA binding motif protein 8B
- RNA-binding motif protein 8A
- RNA-binding protein Y14
- Ribonucleoprotein RBM8A
背景信息 Y14 (RNA binding motif protein 8A or RBM8A), an RNA-binding protein, contains a conserved RNA-binding motif. Y14 is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein. This gene also uses multiple polyadenylation sites. 产品信息 格式 Purified 控制 - HeLa cell ysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-Y14 Antibody, clone 1F12 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect Y14 also known as Binder of OVCA1-1, RNA binding motif protein 8A, Ribonucleoprotein RBM8A. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in immunoprecipitation. Kashima, I.,, et al. (2006). Genes & Dev. 20:355-367.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in immunocytochemistry. Kataoka, N., et al. (2000). Molecular Cell 6(3):673-682.生物信息 免疫原品种 Purified human Y14. 表位 Unknown 克隆 1F12 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Y14. 同种型 IgG1κ 品种反应性 MouseHumanRat Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005096.1
Entrez基因汇总 This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites. [provided by RefSeq] 基因符号 - ZRNP1
- RBM8A
- BOV-1
- BOV-1A
- BOV-1B
- BOV-1C
- MDS014
- RBM8B
- Y14
- ZNRP
纯化方法 Protein G purfied UniProt编号 - Q9Y5S9
UniProt汇总 FUNCTION: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.
SUBUNIT STRUCTURE: Heterodimer with MAGOH. Part of the exon junction complex (EJC) core complex that contains CASC3, EIF4A3, MAGOH and RBM8A. Found in a mRNA splicing-dependent EJC, at least composed of ACIN1, CASC3, DEK, EIF4A3, MAGOH, NCBP1, NCBP2, PNN, NXF1, RBM8A, RENT2, RENT3A, RENT3B, RNPS1, SAP18, SRRM1 and THOC4. Found in a post-splicing complex with NXF1, RBM8A, RENT1, RENT2, RENT3A, RENT3B and RNPS1. Interacts with BAT1, MAGOH, OVCA1, RENT3B, RNPS1, SRRM1 and THOC4. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with WIBG/PYM; the interaction is direct and leads to dissociate the EJC from spliced mRNAs. Associates with polysomes.
SUBCELLULAR LOCATION: Nucleus. Nucleus speckle. Cytoplasm. Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
TISSUE SPECIFICTY: Ubiquitous.
SEQUENCE SIMILARLITIES: Belongs to the RBM8A family.
Contains 1 RRM (RNA recognition motif) domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/ml of this antibody detected Y14 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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